Post-translational Modification Analysis
•Post-translational modification (PTM) refers to the covalent, usually
enzymatic modification of proteins, and protein process during or after
protein biosynthesis. Protein post-translational modification (PTM) increases
the functional diversity of the proteome by the modifying proteins with
functional groups, such as phosphate, acetate, amide groups, or methyl
groups, and influences almost all the aspects of normal cell biology and
pathogenesis. It plays a key role in many cellular processes such as cellular
differentiation, protein degradation, signaling and regulatory processes,
regulation of gene expression, and protein-protein interactions. The
modifications genererally include phosphorylation, glycosylation,
ubiquitination, nitrosylation, methylation, acetylation, lipidation and
proteolysis and influence almost all aspects of normal cell biology and
pathogenesis. Therefore, characterization of PTM, including the modification
categories and modified sites, is critical in the study of cell biology and
disease diagnostics and prevention.
