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contains 3 page ACHARYA N.G RANGA AGRICULTURAL UNIVERSITY FACULTY GF AGRICULTURAL ENGINEERING & TECHNOLO &, Tech, (Food Technology) SEMESTER FINAL THEORY EXAMINATIONS FIRST YEAR - FIRST SEMESTER 2017-18 FSOA 131 GENERAL MICROBIOLOGY (New Syllabus) Marks: 40 Time :30 min Part A t Fill in the blanks with suitable answer of phrase 20x1=20M 1. Anton Vor Leeuwenhoek observed minute movi ing objects ted the finding in Bou) Non-nutritive, Inert, lological media melts at ‘Systema naturae’ is written by J tree The classical Golden era of microbiology is { Li Chromatinic area is rich in ria The state in which Cytoplasm exists is ¥ Boor! RO ee 5 The method of isolation where an organism is is: ure form by serie dilution is called as Cowal di tutte Exponential phase is also called as Woy pie Bacteria that retain the crystal violet color and so appear deep 11. The process in which one creature destroys the life of another in order to sustain its own is called as fwdtibing'« = 12, Porcelain filters were discovered by thames hare besland 13. The process in which the viral DNA is removed from the host's ‘thromasome and in which the lytic cycie occurs is called as__f pis vusemes edluchion 14. The microorganisms that are involved in the Manufacture of foods such as bread, beer, wines, vinegar are for hovo mu fA teow) 15. Fusion of somatic or vegetative cells is called as Somatic ftusla 16. To prevent Opthaimia neonatorum, a gonocaccal infection of eyes few drops of a ‘Solution Is added in the eyes of new born babies is__\)+ nj liv al! are referred to 38 20. Marine bacteria attached to particulate materials _— fig be cio! ee 20x0.5 = 10M {Underline the most appropriate answers: from the given choices. Pasteur institute Is found at : a) Geneva b) Paris c) Rome d) London Theory of biogenesis was proposed by a) Louis Pasteur b) John Needham c) [Lazzaro Spallanzani d) Kurt Lewis ‘Cantagium vivum Aluidurn’ is a) Algae b) Bactena c) Yeast d) Virus Lysozyme Is discovered by 2 a) Alexander Flemming b) Paul Ehrich ¢) Edward Jenner d), Joseph Lister ‘Micrographie' is a : a) Microscope b) Growth curve ¢} Book “Dichroic mirror’ is present in @) Eluormscagt microscope. b) c) Transmission Electron Microscope d) Electron microscope All the cells ina culture were ta be in the same stage of the division cycle in a) ‘Synchronous growth b) Zig zag growth c) Nermal growth d) Abnormal growth A ee of colorimeter can be used for turbidimetric measurements of cell a) Nass c) Number Transverse binary fission occurs in a) Streptococcus faecalis 7 b) Bacillus subtilis c) Rhodopsevdomonas acidophilay d), Presthecobacter fusiformis Yeast extract |s a rich source of Fi a) Vitamin A : b) Vitamin B c) Vitamin C d) _ Vitamin Dy Double stranded DNA contains is present in the phages a) Aphage ‘ b) B phage c) Cphage d) Allofthe above _- ‘The content of genetic information per virion per strand of nucleic acid varies from d) Microorganism Phase-Cantrast Microscope b) Shape d) Color b) 3-300 kilo bases — d) 3-30 kilo bases b) Lipids d) Crude fibre ed in the phenol coefficient method Is b) Coll microbiology pi - for the growth a the field of 6M This Ee seotine Spages " ACHARYA N.G. RANGA AGRICULTURAL UNIVERSITY FACULTY OF AGRICULTURAL ENGINEERING & TECHNOLO‘ av B.Tech. (FOOD TECHNOLOGY) SEMESTER FINAL THEORY EXAMINATIONS FIRST YEAR - FIRST SEMESTER 2018-19 Time: 30 min GENERAL MICROBIOLOGY Marks: 40 PART-A go K1=20M |. Fill in the blanks with suitable word(s) 1, The scientist that discovered the process of pasteurization Is _{|-> feta fo that are UN ire 2. Phase contrast microscope is used to study the microorganisms 3: Teichoic acids are specifically present in cell walls of _ Aus. 4, German Zoologist E.H. Haeckel (1886) suggested kingdom ___ Pe, 5. The study of fungl is called as ey Pay 6. The father of Microbiology is Antony van Lo ueterbuot — 4 = 7, The population of cells that arise Roja single cell is called as 8 Bacterial conjugation was first described by Sa ®. Bacteria arranged in clusters is called as terial flagellum 40. The part that lies between the filament and basal body of the bar a 41. The bacterial cells are lined side by side like match sticks in li arrangement. 42. The polysaccharide used to solidify bacterial media is f g ah aS 43, Rod shaped bacteria are termed as_S[[iv\ 1. gph citurs FA. An ebamative name forpi'ts__cbrabvine | Stequloctiles 45. The ability of a microscope to distinguish two closely related points is called as ‘ont A ort Chapt the 46. Treatment of disease by using chemicals is called as Vere biirmin 17. Temperature for autoclaving is 48, Of the natural sources, most types of the microorganisms are found in Example of baker's yeast is 20. Roll-tube technique ts used for the isolation of A.Stringent anaerobes C.Stringent aerobes: Ti, Match the items of Column A with Colum Column A Antony Von Leeuwenhoek Louls Pasteur R.H Whittaker Haeckel ; dohn Needham i Joseph Lister [ Iwanowski ; Elie Metchincoft i) Paul Echrilich Edward Jenner 1V. Match the items of Column A with Column B given in tha following. Column A Protozoa Spores Gell count Disinfectant Lyophilization Cell wall Vibriod Induction Yeast extract Pleamorphic { nn B given in the following: 23. Spontaneous generation rs B.Thermophilic anaerabes D:Thermophilic aerobes 40x0.5=5M Column & 1. Five kingdom concept Protista Serial dilutions ‘Swan neck 2. 3. 4 5. Animal cules 6. Chemotherapy and antibiotics 7.Cow pox vaccination 9. Tobacco mosaic virus 10, Phagocytosis 44. Petriplates 42, Leaf Wharl disease 10 «0.5 Column B . BVitamins Curved Lytic cycle Eukaryote . Variety of shapes Electronic particle counter Metabolically dormant . Peptidoglycan 9. Sodium Hypochtorite 10.Freeze drying 11.Prokaryote 12,Spherical on oomrkon EFT MESES COE SEPM ACHARYA NG, RANGA AGRICULTURAL UNIVERSITY FACULTY OF AGRICULTURAL ENGINEERING & TECHNOLOGY B.Tech. (FOOD TECHNOLOGY) SEMESTER FINAL THEORY EXAMINATIONS FIRST YEAR - FIRST SEMESTER 2018-18 FSQA 131 GENERAL MICROBIOLOGY Time: 2 hrs. Marks: 60 PART: B ‘A. i 3. b) “ANSWER ANY FIVE QUESTIONS. Draw a neat labeled diagram of bacterial cell and enlist all the external . ‘ALL QUESTIONS CARRY EQUAL MARKS, a) and internal organelles f b) What are the contributions made by the scientist Robert Koch in the field of microbiology? J 4) Write about the importance af industrial microbigteay b) Give an account of nut tional classificalyt of bacteria with sultable examples 4 a) What are the warlous methods fone Describe. ray b) | Write an account on general characteristic features of algae a) Differentiate between prokaryotes and eukaryotes: b) Whatare the ideal features of an antimicrobial chemical agent? a) Describe in detail the production of mushrooms, b) What are the modes of reproduction of bacteria? Mlustrate. a) Describe the industrial uses of yeast. b) Draw.a neat labeled diagram of a simple microscope. Write a short note on the following. 4x3 a) Pure culture b)} Ribosomes ¢) Fluorescent microscopy d) Protozoa a) Whatare the various methods of preservation of microbial cultures? Explain the nutrient transport phenomenon by facilitated diffusion. 6M 6M 6M 6M 6M BM 6M 6M 6M 6M 6M 6M = 12M 6M 6M F I. Underline the appropriate answer 1, Petrofl -Hausser cou ® ‘@) Bacterial Count 2. The principal constituent of ce i a) Chitin b) Pep ©) 3. The phenomenon of inhibiting f a) Gactencita! b | , 4 Mois! hea! method is follow a) Autoclave e 5 One of the followin a} Compound mi c) Electron Microscope p 4. The cryoprolective used 10 1 12. 13. 14 lyophilization/freeze drying a) Dimethy! sulfoxide ¢) Sodium laury! sulfate During the fetiowing growth phases, maximum gro @) Lag phase b) Log phase c) Decline phase The solidifying agent is extracted from @) Green algae b) Brown algae c) Red aigas d) Blue algae ‘The type of pili responsible for the formation, aifugation tube, ¢ conjugation is a) Normal pili b) Sex pili & fagella ‘ The capsid symmetry exhibited B) Helical b) Circe Golden age of Microbiology is a} 1980-1990 b} 1880-1900 The bacteria arranged in spherical shape are termed as c) Gocci @) Bacilli b) Spiral Avculture that represents only one species 0! a) Mixed culture ©) Pure culture According to Bergey's Manual of Systematic Bacteriology, the prokaryotes that lack a cell wall is grouped under a) Division: Mendosicutes ¢) Division: Gracilicutes ‘Surface to volume ratio in bacteria is a) High ¢) Neither high or low The structures that provide buoyancy for the bacteria that live in aquatic habitats are c) PHB granules d) Volutin granules a) Gas vacuoles b) Episome The dyes which contain negative charge on the ions are calied as b) Hydrogen ¢) Hydrech neg of the phages is t ¢} Complex qi c) 1890-1890 f organism is called as b) Synchronous culture d) Asynchronous culture h of bacteria d) Vibrio b) Division: Firmicutes d) Division: Tenericutes b) Low id) Very tow b) Neutral dyes d) Acidic dyes 18, The Principle 8) Protein 19 Scientist who pas dispraving th 8} Franze Schulze a the srnallest distance distinguishabla as two separ a} Magnification ¢) Limit of Reso ution { electron mcr 000% ) 24. The magnification ra a} 1000-2000% b 22. Higher Protista includes a) Algae b) Fungi smic membi b) Mesosomes 23. The infoldi a) Ribosome F 24 Cures are maintained by periodic tran t &) Sub cult c) Lyophitisat 26. Roll-tube technique is used a) Stringent aerobes \_b) Micro mostly present in the cell walls of c) Fung! 26. Teichoic acids @) Gram +ve bacteria) Gram -ve bacteria 27. Porcelain fitters are used by @) Robert Brown b) Jchn Needham = c) Robert Koch 28 The culture in which step wise increase in population occurs is kne a) Batch culture b) Fed-batch culture ¢) Synchronous culture d) Continuous 29. The part that lies between the filament and basal body of the bacterial fi a) P- Ring b) M-Ring c) Hook d) Pi 30. Yeas! extract is a rich source of a) Vitamin C b) Vitamin DB ¢) Vitamin A @) Vitamin B 40x0.5=5M til. Match the following: Paul Echrilich [ ] 1. Phagocytosis Elie Metchincoff [ } 2. Tobacco mosaic virus E.H. Haeckel { ] 3. Serial dilutions Edward Jenner { ] 4. Rhodopseudomanas acidophila Variety of shapes { ] 5. Gram staining Joseph Lister { ] 6. Chemotherapy and antibiotics ‘hwanowski { 2 ] 7. Cow pox vaccination Budding [ 4 } 8. Boiled beef broth Christian Gram [ 1 _& Three kingdom concept Lazaro Spaltanzani { ] 10. Pleomorphic 
