Prokaryote Characteristics and Structures, Exams of Microbiology

Information on the characteristics and structures of prokaryotes, including their lack of a nucleus and organelles, small size, and simple structure. It also covers external structures such as glycocalyces, flagella, and fimbriae, as well as cell walls and cytoplasmic membranes. Additionally, the document discusses the toxic forms of oxygen and the classification of organisms based on oxygen and nitrogen requirements.

Typology: Exams

2022/2023

Available from 10/09/2023

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Bellas Microbiology Exam II
Prokaryote Characteristics - ANS: - No membrane surrounding their DNA; no nucleus
- Lack organelles - internal membrane-bound structures
- Small; ~1.0 μm in diameter (RBC ~10 μm)
- Simple structure
QUIZ: Prokaryote Types - ANS: Bacteria & Archaea
QUIZ: Prokaryote External Structures (3) - ANS: Glycocalyces, Flagella and Fimbrae &
Pilli
QUIZ: Gycocalyx - ANS: "Sugar cage" - Polysaccharides
- Capsule & Slime Layer
QUIZ: Capsule - ANS: Hard glycocalyx with consistent structure around prokaryote
QUIZ: Slime Layer - ANS: Inconsistent outsider layer loosely attached to cell surface;
water soluble; protects cells form druing out; sticky layer that allows prokaryotes to
attache to surfaces
QUIZ: Flagella - ANS: Long, whiplike structures that extend beyond surface of cell and
is responsible for movement
QUIZ: Flagella Structure - ANS: - Composed of filament, hook, and basal body
- Flagellin protein (filament) arranged in chains and forms helix around hollow core
- Base of filament inserts into hook
QUIZ: Flagella Function - ANS: - Rotation propels bacterium through environment
- Rotation can be clockwise or counterclockwise; quickly reversible
- Prokaryotes move in response to stimuli (taxis)
QUIZ: Runs - ANS: movement of cell in singel direction for some time; increase with
favorable stimuli - chemotaxis or phototaxis
QUIZ: Tumbles - ANS: - abrupt, random, changes in direction; increase with
unfavorable stimuli: negative chemotaxis - away fr chemical or negative phototaxis -
away fr sunlight
- no steering mechanism
QUIZ: Fimbrae & Pili - ANS: Nonmotile extensions of prokaryotes
QUIZ: Fimbrae - ANS: - Sticky, proteinaceous, bristlelike projections
- Used by bacteria to adhere to one another, to hosts, and to substances in environment
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- May be hundreds per cell and are shorter than flagella
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Bellas Microbiology Exam II

Prokaryote Characteristics - ANS: - No membrane surrounding their DNA; no nucleus

  • Lack organelles - internal membrane-bound structures
  • Small; ~1.0 μm in diameter (RBC ~10 μm)
  • Simple structure QUIZ: Prokaryote Types - ANS: Bacteria & Archaea QUIZ: Prokaryote External Structures (3) - ANS: Glycocalyces, Flagella and Fimbrae & Pilli QUIZ: Gycocalyx - ANS: "Sugar cage" - Polysaccharides
  • Capsule & Slime Layer QUIZ: Capsule - ANS: Hard glycocalyx with consistent structure around prokaryote QUIZ: Slime Layer - ANS: Inconsistent outsider layer loosely attached to cell surface; water soluble; protects cells form druing out; sticky layer that allows prokaryotes to attache to surfaces QUIZ: Flagella - ANS: Long, whiplike structures that extend beyond surface of cell and is responsible for movement QUIZ: Flagella Structure - ANS: - Composed of filament, hook, and basal body
  • Flagellin protein (filament) arranged in chains and forms helix around hollow core
  • Base of filament inserts into hook QUIZ: Flagella Function - ANS: - Rotation propels bacterium through environment
  • Rotation can be clockwise or counterclockwise; quickly reversible
  • Prokaryotes move in response to stimuli (taxis) QUIZ: Runs - ANS: movement of cell in singel direction for some time; increase with favorable stimuli - chemotaxis or phototaxis QUIZ: Tumbles - ANS: - abrupt, random, changes in direction; increase with unfavorable stimuli: negative chemotaxis - away fr chemical or negative phototaxis - away fr sunlight
  • no steering mechanism QUIZ: Fimbrae & Pili - ANS: Nonmotile extensions of prokaryotes QUIZ: Fimbrae - ANS: - Sticky, proteinaceous, bristlelike projections
  • Used by bacteria to adhere to one another, to hosts, and to substances in environment

  • May be hundreds per cell and are shorter than flagella
  • Serve an import function in biofilms QUIZ: Pili - ANS: - Long hollow tubules composed of pilin
  • Bacteria typically only have one or two per cell
  • Join two bacterial cells and mediate the transfer of DNA from one cell to another (conjugation or sex pili); ~ 10K base pairs QUIZ: Prokaryote Cell Walls - ANS: - Provides structure and shape and protects cell from osmotic forces --> most bacteria live in hypotonic (low salt) solutions; keep H20 fr moving into bacteria & burst it
  • not in animals; can target cell wall of bacteria with antibiotics QUIZ: Bacteria Cell Walls - ANS: - Most have cell wall composed of peptidoglycan; a few lack a cell wall entirely
  • Peptidoglycan composed of sugars, NAG and NAM (mostly sugar w/ peptide change)
  • Chains of NAG and NAM attached to other chains by tetrapeptide crossbridges (scaffolding type structure) -Amino acids tie bridges together
  • Two basic types of bacterial cell walls - Gram-Positive & Gram-Negative QUIZ: Gram-Positive - ANS: - Relatively thick layer of peptidoglycan
  • Contains unique polysaccharides called teichoic acids
  • Some covalently linked to lipids, forming lipoteichoic acids that anchor peptidoglycan to cell membrane (phospholipid bilayer)
  • Retains crystal violet dye in Gram staining procedure; appear purple
  • Acid-fast bacteria contain up to 60% mycolic acid; helps cells survive desiccation QUIZ: Gram-Negative - ANS: - Have only a thin layer of peptidoglycan (can't adhere to crystal violet therefore appears pink following Gram stain)
  • Have a outer membrane composed of phospholipids, channel proteins (porins), and lipopolysaccharide (LPS) --> this part is extremely toxic QUIZ: Lipopolysaccharide (LPS) - ANS: - Union of lipid with sugar
  • Also known as endotoxin (lipopolysaccharide)
  • Lipid portion known as lipid A (extremely poisonous)
  • Released from dead cells when cell wall disintegrates
  • May trigger fever, vasodilation, inflammation, shock, and blood clotting
  • Can be released when antimicrobial drugs kill bacteria QUIZ: Archael Cell Walls - ANS: - Do not have peptidoglycan
  • Cell walls contain variety of specialized polysaccharides and proteins QUIZ: Prokaryotic Cytoplasmic Membrane - ANS: Phospholipid Bilayer --> lipids and associated proteins QUIZ: Prokaryotic Membrane Function - ANS: - Controls passage of substances into and out of the cell; selectively permeable
  • Do not rotate, but undulate rhythmically QUIZ: Cilia - ANS: - Shorter and more numerous than flagella
  • Composed of tubulin in "9+2" (9 microtubules or doublets and central pair of microtubules) and "9+0" arrangements
  • Coordinated beating propels cells through their environment and used to move substances past the surface of the cell QUIZ: Eukaryotic Cell Walls - ANS: - Fungi, algae, and plants have cell walls but no glycocalyx
  • Composed of various polysaccharides QUIZ: Plant Cell Walls Composition - ANS: Cellulose QUIZ: Fungal Cell Walls - ANS: Cellulose, chitin &/or glucomannan QUIZ: Algal Cell Walls - ANS: Cellulose, agar, cargeenan, cilicates, algin, calcium carbonate or combination of these QUIZ: Eukaryotic Cell Membranes - ANS: - All eukaryotic cells have cell membrane
  • Is a fluid mosaic of phospholipids and proteins
  • Contains steroid lipids to help maintain fluidity - stabilize membranes
  • Controls movement into and out of cell - use diffusion, facilitated diffusion, osmosis, and active transport -->Endocytosis; phagocytosis if solid substance and pinocytosis if liquid substance (not all eukaryotes) --> Exocytosis enables substances to be exported from cell removes waste QUIZ: Microbe Nutrients - Based on - ANS: - Source of Carbon
  • Chemical or Light use as a source of energy QUIZ: Definitions as Source of Carbon - ANS: Autotrophs vs. Heterotrophs QUIZ: Autotrophs - ANS: Uses an inorganic carbon source (not hooked up to carbon) QUIZ: Heterotrophs - ANS: catabolizing reduced organic molecules (proteins, carbohydrates, amino acids and fatty acids) QUIZ: Definitions as Chemical or Light Use - ANS: Chemotrophs (acquire energy from redox reactions involving inorganic/organic chemicals vs. Phototrophs (use light as energy source) QUIZ: Four Basic Groups of Organisms - ANS: - Photoautotrophs
  • Chemoautotrophs
  • Photoheterotrophs
  • Chemoheterotrophs

QUIZ: Four Toxic Forms of Oxygen - ANS: - Singlet Oxygen

  • Superoxide Radicals
  • Peroxide Anion
  • Hydroxyl Radical

-->Aerobes also use antioxidants such as vitamins C and E to protect against toxic oxygen products QUIZ: Singlet Oxygen - ANS: - molecular oxygen with electrons boosted to higher energy state; when electrons move back to stable state, release excess energy

  • occurs during photosynthesis so phototropic organisms have carotenoids that remove the excess energy of singlet oxygen QUIZ: Superoxide Radicals - ANS: - some form during incomplete reduction of oxygen in aerobic and anaerobic respiration --> So reactive that aerobes produce superoxide dismutases to detoxify them; we create enzymes that detoxifies superoxide radicals produced --> Anaerobes lack superoxide dismutase and die as a result of oxidizing reactions of superoxide radicals formed in presence of oxygen; anaerobes don't have the enzyme QUIZ: Peroxide Anion - ANS: - formed during reactions catalyzed by superoxide dismutase and other reactions --> Aerobes contain either catalase or peroxidase to detoxify peroxide anion --> Obligate anaerobes either lack both enzymes or have only a small amount of each QUIZ: Hydroxyl Radical - ANS: - results from ionizing radiation and from incomplete reduction of hydrogen peroxide (not formed often) -->Most reactive of the 4 toxic forms of oxygen -->Not a threat to aerobes due to action of catalase and peroxidase QUIZ: Classification of Organisms Based on Oxygen Requirements - ANS: - Aerobes
  • Anaerobes
  • Facultative Anaerobes
  • Aerotolerant anaerobes - do not use aerobic metabolism but have some enzymes that detoxify oxygen's poisonous forms O2 doesn't hurt/help
  • Microaerophiles - aerobes that require oxygen levels from 2-10% and have a limited ability to detoxify hydrogen peroxide and superoxide radicals small amounts of O2 (air has ~20% O2) QUIZ: Aerobes - ANS: undergo aerobic respiration QUIZ: Anaerobes - ANS: do not use aerobic metabolism

QUIZ: Two Common Isolation Techniques - ANS: Streak Plates & Pour Plates QUIZ: Streak Plates - ANS: Sample from initial table w mixed bacteria; drag across to different areas in petri so that gets more and diluted; after incubation can identify different cultures and use a sterile loop to pick up a particular organism QUIZ: Pour Plates - ANS: Create progressively diluted samples using 1/10 dilutions up to 1-10 dilutions; place sample of diluted solution into petri; incubate; isolate bacteria w/ sterile loop QUIZ: Six Types of General Culture Media - ANS: - Defined, Complex, Selective, Differential, Anaerobic & Transport QUIZ: Defined Media - ANS: Defined recipe for its making QUIZ: Complex Media - ANS: Has recipe but something is not chemically precise QUIZ: Selective Media - ANS: selects for growth of some organisms - i.e., using acid to encourage fungi growth and kill bacteria QUIZ: Differential Media - ANS: distinguishing b/t different stains; changes color with pH changes QUIZ: Phases of Microbial Growth - ANS: Lag, Log, Stationary & Death QUIZ: Lag Phase - ANS: Adjust to new surroundings QUIZ: Log Phase - ANS: maximum growth rate given situation QUIZ: Stationary - ANS: nutrient depletion accumulation of wastes (chemostat) - runs out of food or waste products produced suppresses growth QUIZ: Death - ANS: Cells die faster than produced QUIZ: Methods for Measuring Microbial Growth - ANS: Direct Vs. Indirect QUIZ: Direct Method - ANS: Standard Plate count, Viable Plate counts, Membrane Filtration, Microscopic counts, Electronic counters, Most Probable Number QUIZ: Standard Plate Count - ANS: count bacteria on plate QUIZ: Viable Plate Count - ANS: when bacteria is too concentrated, take series of solutions (serial solutions) and based on dilution compute calculation to determine #

QUIZ: Membrane Filtration - ANS: when bacteria is too dilute, filter sample and culture from membrane QUIZ: Microscopic Counts - ANS: utilizes slides with grids; calculate knowing precise volume of fluid w/in slides; can't distinguish b/t dead & live cells QUIZ: Indirect Method - ANS: Metabolic Activity, Dry Weight, Turbidity QUIZ: Metabolic Activity - ANS: may know that bacteria produces vit K, then measure how much Vit K produced QUIZ: Dry Weight - ANS: weigh organism; more weight more bacteria QUIZ: Turbidity - ANS: cloudiness; use spectrophotometer to read cloudiness; sends light thru sample and measures % light that passes through; quick and efficient QUIZ: Symbiosis - ANS: to live together QUIZ: Symbiotic relationships - ANS: the relationship b/t microorganisim & host QUIZ: Types of Symbotic Relationships - ANS: Mutualism, Commensalism, Parasitism QUIZ: Mutalism - ANS: beneficial - Microbe & Infected Organism both benefit (ex. Bacteria in colon) QUIZ: Commensalism - ANS: beneficial - Microbe & Infected Organism both benefit (ex. Bacteria in colon) QUIZ: Parasitsim - ANS: Microbe benefits, infected organism is harmed—causes disease (tuberculosis bacter in human lung) QUIZ: Types of Normal Microbiota (2) - ANS: Resident & Transient QUIZ: Resident Microbiota - ANS: - Part of normal microbiota throughout life (always there)

  • Most are commensal (benefits from place to live) QUIZ: Transient Microbiota - ANS: - Remain in body for only hours to months before disappearing
  • Found in the same regions as a resident microbiota
  • Cannot persist in the body -Competition from other microorganisms (resident bacteria); Elimination by the body's defenses cells (attack unfamiliar); Chemical or physical changes in the body (on medication, recovering, stress, etc.) QUIZ: Acquisition of Normal Microbiota - ANS: - Development in the womb is generally free of microorganisms (axenic) (fetus not exposed to microbes)

QUIZ: Virulence Factors of Infectious Disease - ANS: Pathogenicity & Virulence QUIZ: Pathogenicity - ANS: ability of a microorganism to cause disease (can make mildly/deathly sick) QUIZ: Virulence - ANS: - degree of pathogenicity - virulence factors contribute to an organisms virulence -->Adhesion factors -->Extracellular enzymes -->Toxins -->Antiphagocytic factors QUIZ: Extracellular Enzymes - ANS: - Enzymes secreted by the pathogen

  • Dissolve structural chemicals in the body
  • Help pathogen maintain infection, invade further, and avoid body defenses QUIZ: Toxins - ANS: - Chemicals produced by the pathogen
  • Harm tissues or trigger host immune responses that cause damage
  • Toxemia refers to toxins in the bloodstream that are carried beyond the site of infection
  • In blood, circulating in body - systemic effects QUIZ: Two Types of Toxins - ANS: Exotoxins (made by body) & Endotoxins (part of bacteria) QUIZ: Antiphagocytic Factors - ANS: prevent phagocytosis by the host's phagocytic cells
  • Bacterial capsule - Often composed of chemicals found in the body and not recognized as foreign; can be slippery making it difficult for phagocytes to engulf the bacteria
  • Antiphagocytic chemicals QUIZ: Antiphagocytic Chemicals - ANS: - Some prevent fusion of lysosome and phagocytic vesicles
  • Leukocidins directly destroy phagocytic white blood cells QUIZ: 3 Types of Reservoirs of Infection - ANS: Animal Reservoir, Human Carriers & Nonliving Reservoir QUIZ: Animal Reservoir - ANS: Zoonoses - diseases that are naturally spread from their usual animal host to humans
  • contact w/ animal waste, improperly prepared meat & bloodsucking arthropods QUIZ: Human Carrier - ANS: - Infected individuals who are asymptomatic but infective to others (source)
  • Some individuals will eventually develop illness while others never get sick

QUIZ: Nonliving Reservoir - ANS: Soil, water and food (contaminated) QUIZ: Epidemiology - ANS: Study of where & when diseases occur and how they are transmitted w/in populations QUIZ: Measures of Tracking Occurence of Diseases - ANS: Incidence & Prevalence QUIZ: Incidence - ANS: number of new cases of a disease in a given area during a given period of time QUIZ: Prevalence - ANS: number of total cases of a disease in a given area during a given period of time QUIZ: Nosocomial Infectons - ANS: Infections acquired while in a health care facility QUIZ: Types of nosocomial infections - ANS: - Exogenous- pathogen acquired from the health care environment

  • Endogenous- pathogen arise from normal microbiota due to factors within the health care setting
  • Iatrogenic - results from modern medical procedures QUIZ: