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Information on the characteristics and structures of prokaryotes, including their lack of a nucleus and organelles, small size, and simple structure. It also covers external structures such as glycocalyces, flagella, and fimbriae, as well as cell walls and cytoplasmic membranes. Additionally, the document discusses the toxic forms of oxygen and the classification of organisms based on oxygen and nitrogen requirements.
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Prokaryote Characteristics - ANS: - No membrane surrounding their DNA; no nucleus
QUIZ: Four Toxic Forms of Oxygen - ANS: - Singlet Oxygen
-->Aerobes also use antioxidants such as vitamins C and E to protect against toxic oxygen products QUIZ: Singlet Oxygen - ANS: - molecular oxygen with electrons boosted to higher energy state; when electrons move back to stable state, release excess energy
QUIZ: Two Common Isolation Techniques - ANS: Streak Plates & Pour Plates QUIZ: Streak Plates - ANS: Sample from initial table w mixed bacteria; drag across to different areas in petri so that gets more and diluted; after incubation can identify different cultures and use a sterile loop to pick up a particular organism QUIZ: Pour Plates - ANS: Create progressively diluted samples using 1/10 dilutions up to 1-10 dilutions; place sample of diluted solution into petri; incubate; isolate bacteria w/ sterile loop QUIZ: Six Types of General Culture Media - ANS: - Defined, Complex, Selective, Differential, Anaerobic & Transport QUIZ: Defined Media - ANS: Defined recipe for its making QUIZ: Complex Media - ANS: Has recipe but something is not chemically precise QUIZ: Selective Media - ANS: selects for growth of some organisms - i.e., using acid to encourage fungi growth and kill bacteria QUIZ: Differential Media - ANS: distinguishing b/t different stains; changes color with pH changes QUIZ: Phases of Microbial Growth - ANS: Lag, Log, Stationary & Death QUIZ: Lag Phase - ANS: Adjust to new surroundings QUIZ: Log Phase - ANS: maximum growth rate given situation QUIZ: Stationary - ANS: nutrient depletion accumulation of wastes (chemostat) - runs out of food or waste products produced suppresses growth QUIZ: Death - ANS: Cells die faster than produced QUIZ: Methods for Measuring Microbial Growth - ANS: Direct Vs. Indirect QUIZ: Direct Method - ANS: Standard Plate count, Viable Plate counts, Membrane Filtration, Microscopic counts, Electronic counters, Most Probable Number QUIZ: Standard Plate Count - ANS: count bacteria on plate QUIZ: Viable Plate Count - ANS: when bacteria is too concentrated, take series of solutions (serial solutions) and based on dilution compute calculation to determine #
QUIZ: Membrane Filtration - ANS: when bacteria is too dilute, filter sample and culture from membrane QUIZ: Microscopic Counts - ANS: utilizes slides with grids; calculate knowing precise volume of fluid w/in slides; can't distinguish b/t dead & live cells QUIZ: Indirect Method - ANS: Metabolic Activity, Dry Weight, Turbidity QUIZ: Metabolic Activity - ANS: may know that bacteria produces vit K, then measure how much Vit K produced QUIZ: Dry Weight - ANS: weigh organism; more weight more bacteria QUIZ: Turbidity - ANS: cloudiness; use spectrophotometer to read cloudiness; sends light thru sample and measures % light that passes through; quick and efficient QUIZ: Symbiosis - ANS: to live together QUIZ: Symbiotic relationships - ANS: the relationship b/t microorganisim & host QUIZ: Types of Symbotic Relationships - ANS: Mutualism, Commensalism, Parasitism QUIZ: Mutalism - ANS: beneficial - Microbe & Infected Organism both benefit (ex. Bacteria in colon) QUIZ: Commensalism - ANS: beneficial - Microbe & Infected Organism both benefit (ex. Bacteria in colon) QUIZ: Parasitsim - ANS: Microbe benefits, infected organism is harmedācauses disease (tuberculosis bacter in human lung) QUIZ: Types of Normal Microbiota (2) - ANS: Resident & Transient QUIZ: Resident Microbiota - ANS: - Part of normal microbiota throughout life (always there)
QUIZ: Virulence Factors of Infectious Disease - ANS: Pathogenicity & Virulence QUIZ: Pathogenicity - ANS: ability of a microorganism to cause disease (can make mildly/deathly sick) QUIZ: Virulence - ANS: - degree of pathogenicity - virulence factors contribute to an organisms virulence -->Adhesion factors -->Extracellular enzymes -->Toxins -->Antiphagocytic factors QUIZ: Extracellular Enzymes - ANS: - Enzymes secreted by the pathogen
QUIZ: Nonliving Reservoir - ANS: Soil, water and food (contaminated) QUIZ: Epidemiology - ANS: Study of where & when diseases occur and how they are transmitted w/in populations QUIZ: Measures of Tracking Occurence of Diseases - ANS: Incidence & Prevalence QUIZ: Incidence - ANS: number of new cases of a disease in a given area during a given period of time QUIZ: Prevalence - ANS: number of total cases of a disease in a given area during a given period of time QUIZ: Nosocomial Infectons - ANS: Infections acquired while in a health care facility QUIZ: Types of nosocomial infections - ANS: - Exogenous- pathogen acquired from the health care environment