Proper Phlebotomy Procedures, Exams of Medicine

Various aspects of proper phlebotomy procedures, including specimen handling, patient identification, tourniquet application, needle selection, tube filling, and post-collection steps. It addresses key considerations such as preventing contamination, ensuring patient safety, and maintaining sample integrity. The information provided aims to guide phlebotomists in delivering high-quality, standardized blood collection practices that minimize errors and risks. By understanding the correct protocols, phlebotomists can contribute to accurate laboratory testing and effective patient care.

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2024/2025

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NHA Phlebotomy certification EXAM
1. What temperature should a semen sample be transported? - ANS 36-38 degrees C
2. Which of the following would result in a grossly hemolyzed specimen?
a. Incorrect order of draw
b. Incorrect needle gauge
c. Applying tourniquet too close to the draw site
d. Failure to invert tube - ANS b. Incorrect needle gauge
3. Which of the following is proper procedure after collecting ammonia specimen?
a. Protect from light
b. Keep specimen warm
c. Keep specimen at body temp
d. Place specimen in icewater slurry (or a cup of ice) - ANS d. Place specimen in icewater slurry (or a cup
of ice)
4. The phlebotomist has a request to collect a CBC from a healthy 10 month old. Which is appropriate? -
ANS a. Either heel
5. A phlebotomist is preparing to draw blood from a patient in hospital/ The patient extends his arm and
turns head away. Which consent does this indicate? - ANS a. Implied consent
6. Why should phlebotomist document volume of blood drawn from a patient? - ANS a. Iatrogenic
anemia could be a result fo too much taken
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NHA Phlebotomy certification EXAM

  1. What temperature should a semen sample be transported? - ANS 36-38 degrees C
  2. Which of the following would result in a grossly hemolyzed specimen? a. Incorrect order of draw b. Incorrect needle gauge c. Applying tourniquet too close to the draw site d. Failure to invert tube - ANS b. Incorrect needle gauge
  3. Which of the following is proper procedure after collecting ammonia specimen? a. Protect from light b. Keep specimen warm c. Keep specimen at body temp d. Place specimen in icewater slurry (or a cup of ice) - ANS d. Place specimen in icewater slurry (or a cup of ice)
  4. The phlebotomist has a request to collect a CBC from a healthy 10 month old. Which is appropriate? - ANS a. Either heel
  5. A phlebotomist is preparing to draw blood from a patient in hospital/ The patient extends his arm and turns head away. Which consent does this indicate? - ANS a. Implied consent
  6. Why should phlebotomist document volume of blood drawn from a patient? - ANS a. Iatrogenic anemia could be a result fo too much taken
  1. A phlebotomist has a requisition to draw blood on a patient with severe Down's syndrome. What steps would you take? - ANS a. Speak with the patient guardian before performing venipuncture to enmsure the patient understands
  2. A 69 year old unconscious patient is brought ot the ER. Several blood tests are ordered. The phlebotomist collects blood under - ANS a. Implied consent
  3. Which of the following actions is appropriate when labeling specimens? - ANS a. Label specimens at the time of collection in front of the patient
  4. Before collecting blood on an inpatient, the phlebotomist notices there's no wristband on wrist. There is one on the IV pole. What do you do? - ANS a. Don't draw until nurse puts an armband on patient
  5. Which is appropriate form of identification in inpatient setting? - ANS a. Verbal and wrist band
  6. WBC, RBC, Electrolytes are ordered. Which tubes are used for capillary? - ANS a. Lavender and red
  7. A blood specimen is rejected by lab with QNS as the reason? Why? - ANS a. The specimen was insufficient-not enough blood
  8. Which of the following is best method to prevent internal or external bleeding from venipuncture site after needle is removed? - ANS a. Apply pressure to prevent leakage of blood into the tissues
  9. A physician orders a STAT but phlebotomist forgets to fill out patient ID number. What is appropriate procedure for an improperly labeled specimen when received in lab? - ANS a. Lab rejects the mislabeled specimen
  10. Phlebotomist is performing venipuncture and patient begins to seize. What is correct action? - ANS a. Discontinue the draw
  1. After placing tourniquet on a patients arm, the phlebotomist notices small red spots below the tourniquet. - ANS a. Petechiae
  2. The phlebotomist should examine the antecubital veins of both arms because: - ANS a. The median cubital might be more accessible on one side than the other
  3. What is the appropriate cleanser to clean the meatus for a clean catch urine specimen? - ANS a. Benzalkonium chloride
  4. When should the code on glucose cuvettes or strips be compared to the code on the glucose meter?
  • ANS a. When the patient is tested
  1. Which of the following is appropriate to use when collecting for a chemistry test that requires serum? - ANS a. SST (this is the red gel tube)
  2. A patient state she is a difficult draw and requests a skin puncture. The test is a PTT. Which should you do? - ANS a. Use a syringe and transfer to a light blue tube
  3. Industry standard states needle insertion in the antecubital area should be: - ANS a. 15-30 degree angle
  4. Tubes with which additive should be used for CBC? - ANS a. EDTA (purple tube)
  5. When preparing for transport, which specimen must be placed on ice within 30 minutes after collection? - ANS a. Arterial Blood Gases
  6. Which provides the phlebotomist the best protection when drawing blood from a patient with active TB? - ANS a. N-95 respirator mask
  1. During a draw, the patient becomes unresponsive. What should you do? - ANS a. Check for breathing
  2. Why should a phlebotomist avoid drawing blood from a leg or foot of a diabetic patient? - ANS a. These areas are more susceptible to infection
  3. Why is it important to place a specimen in a biohazard specimen bag outside the laboratory? - ANS a. The biohazard bag prevents a possible exposure incident
  4. As a student. Proper introduction - ANS a. Hi, my name is Sally Smith. I am a student phlebotomist and I'm here to collect blood specimen
  5. When using evacuated tube system (EVS). - ANS a. Position the needle in the same direction as the vein at 30 degree angle
  6. What is additive for a PT/PTT? - ANS a. Sodium Citrate (Blue top)
  7. Blood cultures have been ordered on ICU patient. You would use: - ANS a. Aerobic and anaerobic bottles
  8. A phlebotomist is required to obtain what from a potential donor? - ANS a. Complete medical history
  9. Phlebotomist needs to draw a glucose tolerance test on a patient and notices an empty breakfast plate at bedside. What should you do? - ANS a. Ask the patient when he or she ate last
  10. Which vein should you attempt first? - ANS a. Median cubital
  1. A physician ordered a Tobramycin level to be drawn one hour after dose. The Tobramycin has been ordered for 11am. What should you do - ANS a. Check with the nurse to make sure meds were given (or not given)
  2. phlebotomist notices red spots. What is appropriate action? - ANS a. Apply pressure after collecting During the draw, the the sample
  3. What is a potential consequence of mislabeling a blood bank specimen? - ANS a. A Transfusion reaction
  4. When should a phlebotomist perform external liquid controls for quality control on CLIA waived point of care test? - ANS a. When a new lot number or kit is opened
  5. A 3.3 kg infant has a total blood volume of 240-330ml - ANS
  6. Cleanse in an outward spiral - ANS
  7. What would you use for a hand draw? - ANS a. Butterfly or winged infusion set
  8. Invert anticoagulant tubes to :prevent coagulation - ANS Name the 3 types of blood samples - ANS whole blood/ plasma/ serum What two States require licensure - ANS Louisiana & California When the Phlebotomist collects blood, in which of the patient's zones of interpersonal space is he/she operating? - ANS The patient's Intimate Zone or Space

Define Phlebotomy - ANS An incision into the vein What is the meaning of these acronyms: ASPT, ASCP, and NHA? - ANS ASPT = American Society of Phlebotomy Technicians ASCP = American Society for Clinical Pathology NHA = National Healthcareer Association Define Reference Laboratory: - ANS A reference laboratory is one that specializes in one test or one group of tests, or where more unusual or obscure tests are performed.

. Number the following items to reflect the correct blood flow through the heart and lungs: 1_ Vena Cave (superior/inferior) 7_ Lungs 5_ Pulmonary Valve 6_ Pulmonary Artery 13_ Aorta 9_ Left Atrium 2_ Right Atrium 8_ Pulmonary Veins 4_ Right Ventricle 11_ Left Ventricle 3_ Tricuspid Valve 12_ Aortic Valve 10_ Mitral Valve (aka, Bi-Cuspid) - ANS To whom does the phlebotomist report? - ANS A technical phlebotomy supervisor What two government agencies administer CLIA? - ANS Center for Medicare/Medicaid Services (CMS)__ FDA . Is the Joint Commission a non-profit organization? - ANS No, it's a not for profit organization

. How full should you fill the Sharps Container before using a new container? - ANS ¾ full . What is the name of the multi-purpose fire extinguisher? - ANS ABC To what does the term "aseptic techniques" refer? - ANS Techniques that provide a degree of cleanliness that prevents infection and keeps the phlebotomy environment free of contamination by microorganisms. . A nosocomial infection is: a. Something acquired by healthcare workers. b. Something acquired by visitors to a hospital. c. Something acquired by patients. d. A Hospital Acquired Infection. e. Caused by antibiotic resistant bacteria. f. Both c and d. g. Both a and c. - ANS f. Both c and d. c. Something acquired by patients. d. A Hospital Acquired Infection When is hand hygiene performed? a. When gloves are visibly soiled. b. Before putting on gloves c. After removing gloves d. All of the above e. Both a and b - ANS d. All of the above a. When gloves are visibly soiled.

b. Before putting on gloves c. After removing gloves Before entering an isolation room, what is the first thing the phlebotomist should do? a. Wash hands b. Put on a gown, mask and gloves c. Read the posted instructions d. Obtain permission - ANS c. Read the posted instructions What does the acronym PASS describe and stand for? - ANS It describes the use of a fire extinguisher and stands for: Pull pin, Aim nozzle, Squeeze handle, Sweep from side to side. What does the acronym RACE stand for? - ANS It stands for what a healthcare worker should do if a fire or explosion occurs in the workplace: Rescue, Alarm, Contain, Exit

. Before placing a call on hold, a phlebotomist should: a. record the caller's name. b. check to see if it is an emergency. c. transfer the call to the phlebotomy supervisor. d. tell the caller his or her name. - ANS b. check to see if it is an emergency. A competent professional demeanor is shown by: a. neatness and cleanliness. b. courtesy and cheerfulness. c. pleasantness.

To prevent blood from clotting the specimen must be: d. both A and B c. centrifuged right after collection. d. both A and B - ANS d. both A and B d. both A and B Which of the following pairings is wrong? a. icteric and yellow. b. lipemic and cloudy. c. hemolyzed and red. - ANS d. fasting and cloudy. The main anticoagulant for coagulation studies is: a. ethylenediaminetetraacetic acid (EDTA). b. sodium citrate. c. heparin. d. oxalate. - ANS b. sodium citrate.

. ABO and Rh typing are performed in which laboratory section? a. Hematology b. Blood bank c. Chemistry d. Cytology - ANS b. Blood bank

The activated partial thromboplastin time (APTT) or partial thromboplastin time (PTT) test is performed in: a. coagulation. b. chemistry. c. urinalysis. d. histology. - ANS a. coagulation.

. Which test would a physician order on a patient with a suspected infection? a. Glucose b. Complete blood count (CBC) c. Cholesterol d. Antinuclear antibody (ANA) - ANS b. Complete blood count (CBC) An anemia would be detected by a: a. glucose test. b. blood culture. c. urinalysis. d. complete blood count. - ANS d. complete blood count. . An agency that assures quality care by hospitals is the: a. Joint Commission. b. Centers for Disease Control and Prevention. c. Occupational Safety and Health Administration. d. College of American Pathologists. - ANS a. Joint Commission.

a. source. b. contaminated surface. c. mode of transmission. d. host. - ANS b. contaminated surface. An infection contracted by a patient while in the hospital is termed: a. antibiotic-resistant. b. staphylococcal. c. nosocomial. d. unfortunate - ANS c. nosocomial. The recommended disinfectant for blood and body fluid contamination is: a. sodium hydroxide. b. antimicrobial soap. c. hydrogen peroxide. d. sodium hypochlorite. - ANS d. sodium hypochlorite.

. The term universal precautions refers to: a. universal blood donors. b. mixing organic chemicals under a hood. c. wellness clinics available to everyone. d. treating all specimens as infectious. - ANS d. treating all specimens as infectious. In addition to changing gloves between every patient, phlebotomists should also: a. change their lab coats.

b. disinfect the phlebotomy tray. c. double-bag the specimens. d. wash their hands. - ANS d. wash their hands. To comply with the current Bloodborne Pathogens Standard, phlebotomists must: a. discard sharps only into containers attached to the wall. b. activate the needle protection device before removing the needle from the adapter. c. decontaminate needle adapters between each patient. d. activate the needle protective device and discard the needle and the adapter. - ANS d. activate the needle protective device and discard the needle and the adapter. The code word PASS refers to: a. storage of volatile chemicals. b. operation of a fire extinguisher. c. labeling of hazardous material. d. the presence of radioactive material - ANS b. operation of a fire extinguisher. Alcohol-based hand sanitizers may be used as a substitute for handwashing: a. only in a blood donation center. b. only in certain patient care areas such as oncology or obstetrics and gynecology. c. at any time. d. when the hands are not visibly contaminated with blood or other body fluid. - ANS d. when the hands are not visibly contaminated with blood or other body fluid The prefix that means decreased is: a. hypo-

c. brachial. d. temporal. - ANS b. radial. The blood vessels that carry deoxygenated blood to the heart are: a. arteries. b. alveoli. c. capillaries. d. veins. - ANS d. veins. All of the following are formed elements found in the blood except: a. erythrocytes. b. platelets. c. macrophages. d. leukocytes. - ANS c. macrophages

. The primary role of the thrombocyte is to: a. transport nutrients. b. promote blood clotting. c. phagocytize bacteria. d. produce antibodies. - ANS b. promote blood clotting. . Functions of the blood include all of the following except: a. transport oxygen. b. regulate pulse rate. c. deliver nutrients.

d. transport waste products. - ANS b. regulate pulse rate. The fluid that comprises approximately 55% of the total blood volume is made up of: a. hematocrit. b. plasma. c. tissue fluid. d. serum. - ANS b. plasma. The primary antiseptic for routine venipuncture is: a. iodine. b. chlorhexidine. c. isopropyl alcohol. d. Betadine. - ANS c. isopropyl alcohol. A properly tied tourniquet: a. permits arterial flow and blocks venous flow. b. blocks arterial and venous flow. c. prevents backflow. d. permits venous flow and blocks arterial flow. - ANS a. permits arterial flow and blocks venous flow. The color coding of evacuated tubes provides information about all of the following except the: a. volume of specimen collected. b. type of specimen collected. c. need to invert the tube. d. presence of an anticoagulant. - ANS c. need to invert the tube.