SSCP analysis questions with correct answers
What Does SSCP analysis stand for? Correct Answer-Single strand conformation
polymorphism analysis
What is the simplest and most used method of mutation detection? Correct
Answer-SSCP analysis
Give a simple summary of SSCP analysis procedure Correct Answer-PCR is used
to amplify the region of interest and the resultant DNA is separated as single
stranded molecules by electrophoresis gel.
On what premises does this whole procedure rely on? Correct Answer-1. Single
strand DNA folds differently if it differs by a base.
2. Mutations induce changes in tertiary structure
3. Changes in tertiary structure result in different mobilities
What 3 methods are used to detect the mutations? Correct Answer-Radioactive
detection, staining, fluorescent PCR pairs.
Is it possible to detect whether a mutation will be detected under given conditions?
Correct Answer-Although the tertiary structure of single stranded DNA changes
under different physical conditions, it is not possible
What is the mutation detection sensitivity of PCR-SSCP? Correct Answer-80% in
a single run for fragments shorter than 300bp
T or F: The absence of a new band does not prove that there is no mutation in the
analysed molecule. Correct Answer-True, sensitivity is 80% not 100%
