Staining Methods - Microbiology - Lecture Slides, Slides of Microbiology

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Staining Methods

Types of Staining

• Simple staining
• Differential staining
• Negative staining
• Impregnation method
• Special staining – for certain bacteria,

bacterial spores, parasites & fungi

DIFFERENTIAL STAINS

• Imparts different color to different

bacteria.

• Most widely used differential stains are:

Gram stain & Ziehl Neelsen stain

Gram’s stain

• It differentiates bacteria into two broad

groups : Gram positive Gram negative

• appear violet –appear red

NEGATIVE STAINS

• Produce colored background against which bacteria stand out in contrast.
• Used for bacterial capsule, spirochetes eg India ink, Nigrosin.

IMPREGNATION METHOD

• Cell structures too thin to be seen under microscope are rendered visible by silver impregnation on their surfaces.
• Used for the demonstration of bacterial flagella, spirochetes

Giemsa Stain & Leishman Stain

• For parasites causing Malaria Filaria Leishmaniasis etc.
• Use for staining of peripheral blood smear.

Fluorescent Stain

• Acridine orange
• Fluorescein Isothiocyanate (FITC)
• Auramine rhodamine