DNA Synthesis during Polyomavirus Replication: Mechanisms and Key Proteins, Slides of Human Resource Management

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Initiation of DNA Synthesis

Two LT hexamers bind Binding distorts early palindrome unwinding origin Binding of Rpa occurs 31

DNA Synthesis Initiates at a

Unique Origin

RE Site Ori RE Site Ori RE Site How do you know that replication is bidirectional? 32

The Leading Strand Is Easy

Presynthesis complex pol α, T and Rp-A

Rf-C binds 3’OH along with PCNA and pol δ -Rf-C a clamp loading protein -Allows entry of PCNA on DNA -Causes release of pol α

Form sliding clamps along DNA

Continuous copying of parental strand 34

The Lagging Strand - Not So Easy

1st primer and Okazaki fragment made by pol α-primase complex

DNA is copied from the replication fork toward the origin

Multiple initiations are required to replicate the template strand

Both leading and lagging strands move in the same direction!

Which moves, the DNA or the complex? -Template has to move, otherwise......? 35

Cellular Proteins Required for

Polyomavirus DNA Replication

37

DNA Synthesis by Polyomaviridae is

Bidirectional

38 Leading strand presynthesis complex

40

DNA Synthesis by Polyomaviridae is

Bidirectional