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Erythropoietin
treats anemia in kidney patients
TERM 2
Clotting Factors
DEFINITION 2
Factor VIII
TERM 3
Insulin
DEFINITION 3
designer
TERM 4
Transgenic Plants and Animals
DEFINITION 4
treat genetic diseases
TERM 5
Transgenic Plants have:
DEFINITION 5
bt toxins
Transgenic animals have:
lactoferrin
TERM 7
DNA Cloning
DEFINITION 7
replicating foreign DNA in a host cell
TERM 8
Vectors (DNA
Cloning)
DEFINITION 8
take up foreign DNA and can replicate in the host cell
TERM 9
Restriction Endonuclease
DEFINITION 9
naturally occurring enzymes that cleave at specific
palindromic sequences; EcoRI
TERM 10
EcoRI
DEFINITION 10
staggered cut (restriction endonuclease)
Expression Vector: Replication Start
Ori
TERM 17
Expression Vector: Transcription Start
DEFINITION 17
Promoter - Pribnow box (-10)
TERM 18
Expression Vector: Transcription Stop
DEFINITION 18
p (rho) site - GC hairpin oligoU
TERM 19
Expression Vector: Translation
Start
DEFINITION 19
Shine-Dalgarno + AUG (in eukaryotic DNA)
TERM 20
Polymerase Chain Reaction (PCR)
DEFINITION 20
amplifying DNA without having to clone in host cell
Uses of Polymerase Chain Reaction (PCR)
Diagnosis - HIV, tuberculosis
Paternity Tests
Criminal investigations
TERM 22
DNA Fingerprinting
DEFINITION 22
PCR amplification of STRs (short tandem repeats)
GATA - 5-16 repeats
Fluorescent primer
TERM 23
AmpF/STR
DEFINITION 23
Amelogenin - sex
TERM 24
Prince Edward Island
DEFINITION 24
Woman murdered
Near body found a leather jacket with her blood on it and
white cat hair
Estranged husband has white cat named "Snowball"
TERM 25
RNA Interference: Suppression of Gene
Expression
DEFINITION 25
dsRNA inhibits expression of complimentary mRNA
Inject siRNA protein
p53 - decrease mutant, no harm to normal protein
