Performance date / /
Estimation of Serum Glucose
Introduction:
There are different methods to estimate the blood glucose
(1) Orthotoluedene Method
(2) Enzymatic Method
Principle of the Enzymatic Method
In this test, glucose is oxidized to gluconic acid and hydrogen peroxide in the presence
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by the catalytic action of peroxidase (PO) to from a red colored
quinoneimine dye complex. Intensity of the color formed is directly proportional to the
amount of glucose present in the sample.
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Red colour (chromogen)
Sample
Glucose kit is used for the determination of glucose in serum, plasma and CSF. Samples
usually taken are Serum, Heparinised plasma, EDTA plasma.
Procedure
a)
Take Three Test tubes mark them “T” for Test “S” for Standard and “B” for Blank
b) Take 1 ml of Glucose Reagent in all test Tubes
c) Put 10ul serum in Test tube T
d) Put 10ul Standard solution in test tube S
e) Put all test tubes in water bath for 5 min at 370C
f) Measure the absorbance of the standard and samples against the reagent
blank at 546nm wavelength
Calculation
Glucose concentration = Absorbance of
sample
Absorbance of standard
X concentration of standard
