Report sheet
Worksheet 6
Thin-Layer Chromatographic Analysis of Analgesics
NAMES: ClarityD. DATE OF SUBMISSION:
November 13, 2021
1. Provide the purpose of each of the following:
a. TLC plate
The TLC plate is used to identify the molecules on which is more polar and which
is not. Since the TLC plate is coated with the silica gel. Different substances get
to be pulled apart more due its polarity.
b. ethyl acetate
The ethyl acetate is used as a polar solvent in the mobile phase to further
separate polar compounds with the non-polar compounds
c. UV lamp
Since the sample placed on the TLC plate is colorless and doesn’t appear in
normal light, the UV lamp is used to view the spot placed in the plate
d. ceric ammonium molybdate
The cerium ammonium molybdate acts as a stain to the sample for easier
observation of the sample when we can’t easily observe in UV light
2. Answer the following questions briefly, but concisely:
a. Can you use pen as a marker when using TLC plate?
No, you cannot use a pen to mark the TLC plate as it may smear on the plate and
may cause some complications during the chromatography process
b. Why is it necessary to allow the mobile phase to stand for at least 30 minutes inside
the development chamber before placing the TLC plate?
