Using Genes: Lecture Notes - Genetic Engineering | IB 102, Study notes of Biology

Material Type: Notes; Class: Plants, People & Environment; Subject: Integrative Biology; University: University of Illinois - Urbana-Champaign; Term: Unknown 1989;

Typology: Study notes

Pre 2010

Uploaded on 03/10/2009

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Integrative Biology 102: Lecture Outline
Using genes: Genetic Engineering
Lecture Objectives:
1. By the end of the lecture, you should be able to: define the term totipotency
and explain how this characteristic of plants is used in new technology;
2. compare the genetic engineering with traditional plant propagation and
breeding methods.
3. explain the universality of the genetic code and its importance to genetic
engineering.
4. explain, with the use of a diagram, recombinant DNA techniques of moving
desired genes from an organism to the expression of the trait in a plant.
5. discuss the limitations of the genetic engineering.
6. explain how previously unexpressed genes may be expressed through
transgenomics.
Reading: Ch. 15.1-15.4
Terms
recombinant DNA
plasmid
cloning
transgenic
GMO
restriction enzyme
transformation
vector
Agrobacterium tumefaciens
ligase
genetic engineering
selective breeding
gene gun
1. New Technology vs. Traditional Breeding Program
o Common Goal
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Integrative Biology 102: Lecture Outline Using genes: Genetic Engineering

Lecture Objectives:

  1. By the end of the lecture, you should be able to: define the term totipotency and explain how this characteristic of plants is used in new technology;
  2. compare the genetic engineering with traditional plant propagation and breeding methods.
  3. explain the universality of the genetic code and its importance to genetic engineering.
  4. explain, with the use of a diagram, recombinant DNA techniques of moving desired genes from an organism to the expression of the trait in a plant.
  5. discuss the limitations of the genetic engineering.
  6. explain how previously unexpressed genes may be expressed through transgenomics.

Reading: Ch. 15.1-15.

Terms recombinant DNA plasmid cloning transgenic GMO

restriction enzyme transformation vector Agrobacterium tumefaciens ligase

genetic engineering selective breeding gene gun

  1. New Technology vs. Traditional Breeding Program o Common Goal

o Limitations of Traditional Breeding Program

o Advantages of the Genetic Engineering

  1. Terms o recombinant DNA technique

o transformed or transgenic organism

  1. Steps in transforming a plant using Recombinant DNA o Locate & isolate desired genes

o Cut DNA open & remove gene

o Producing products with transformed bacteria

o Producing transformed plants Deliver gene into chromosome  Mitosis

 Cells into tiny plants

o Totipotency

o Tissue Culture

o Individual plants that express the gene

o Gene gun

o Electroporation

o Activation Tagging

  1. Limitations

For the next lecture: Using genes: Applications & Concerns, read 15.6-15.6, and be ready to answer the question:

o What are advantages and disadvantages of using genetically modified foods?