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Wet Mount Preparation Technique, Exams of Nursing

Chamberlain College of NursingNursing

A detailed overview of the steps involved in preparing a wet mount, a common microscopy technique used to observe microorganisms. It covers the essential steps, including cleaning the slide, creating a hydrophobic barrier, applying the specimen, and viewing under a microscope. The document also discusses the advantages of using a wax or hydrophobic pen, the staining properties of gram-negative bacteria, and the considerations around heat-fixing samples. Additionally, it addresses the proper disposal of lab materials and the identification of organisms based on their gram status, shape, and characteristics. This information would be valuable for students studying microbiology, biology, or laboratory techniques, as it provides a comprehensive understanding of the wet mount preparation process and its applications.

Typology: Exams

2023/2024

Available from 09/13/2024

marie-clara
marie-clara 🇺🇸

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Partial preview of the text

Download Wet Mount Preparation Technique and more Exams Nursing in PDF only on Docsity!

Lis t

t t

hefourmainstepsusedtoprepareaWETmount,andindicatewhich(ifany)step

isoptional.

YourAnswer:

  • Cleanslideandmakesuretheslidehascleanvisiblesurface
  • Circleareaonslideforeasylocationofspecimen

• A

p

p p

lyorganism toslide

I

f

f f

rom culture,usesterilel

o

o o

ptospreadontoslide

I

f

f f

rom plate,usesteriletopickcoloniesandmixwiththedropofdisti l edwater

  • Viewundermicroscope

Itisidealtoviewmotilityoftheorganism

Circletheareaforeasylocationofspecimencanbeoptional

1 – Cleanslide 2 –

Circleareaonslidewithwax/hydrophobicpen 3 – A p

lyorganism toslide 4 – Viewundermicroscope

*Note:therearenooptionalstepsinthewetmount.

Whenpreparingaglassslideforadrymount,whatcleaningsolutionwasused?

YourAnswer:

Ethanol 70 %

70 % ethanol

Question 2

2. 5 / 2. 5 pts

2. 5 / 2. 5 pts

Whenperformingawetmoun t

t t

echnique,whatistheadvantageofusingawaxor

hydrophobicpen?

YourAnswer:

itdoesnotle t

tthewatergooutanditk e

eepsitinsidethering

Creatingahydrophobicba r ier(thecircle)helpstok e pthewaterwithinthe

circlesoitdoesnotspi l offoftheslide.

WhatdyedoGram-negativebacteriaprimarilyretain?

YourAnswer:

Safranin

Safranin

WhydoGram-negativebacteriastainred?

YourAnswer:

theyhaveathinpeptidoglycanlayer,sothealcoholwashesawaythecrystalvioletand

thenbecauseofremainingsafranintheya p

p p

earinthepinkcolor

Gram-negativebacteriahaveathinpeptidoglycanlayerintheirce l wa l .During

thealcoholrinsestep,thece l wa l ca n otretainthecrystalvioletdye.Itwi l be

washedaway.Thece l wa l wi l thenabsorbthecounterstain,safranin.

Identifythestainandshapeofthebacteriapicturedbelow.

Question 6

0 / 2. 5 pts

Question 5

2. 5 / 2. 5 pts

Question 4

2. 5 / 2. 5 pts

2. 5 / 2. 5 pts

Whatisonedisadvantageofheatfixingasample?

YourAnswer:

itcausesthebacteriatobeki l edandtheyweca n

n n

ots

e

e e

theirmovement

Theheatfixingprocedureki l sthespecimen.Thisalsopreventsanyobservations

onmotilityandenzymaticproperties.

Whatistheproperwaytodisposeofa l materialsusedduringthelab?

YourAnswer:

weshouldstorechemicalwastesinpropercontainerslikeplasticbo t les

A l materialsmustbeplaceinabiohazardouswastebagandplacedintoan

autoclaveforsterilization.

WhataretheGram status,shape,andidentificationoforganism # 1 from theGram stain

procedure?

YourAnswer:

Gram negative,rodshape

Gram-positive;Co c i(spherical)clusters;Staphaureus

WhataretheGram status,shape,andidentificationoforganism # 5 from theGram stain

procedure?

YourAnswer:

Therewerejus t

t t

hr

e

e e

organism forthegram stain

Gram-positive;Co c i(spherical)chain;Streptoco c us

Question 12

0 / 2. 5 pts

Question

0 / 2. 5 pts

Question 10

0 / 2. 5 pts

2. 5 / 2. 5 pts