A Highly Sensitive Method for Protein Product
Simple Introduction
First of all, let’s start with its definition: Peptide mapping is the main method for the analysis and
identification of protein products and preparations. In addition, there are some other core techniques such as
peptide mass mapping and peptide mapping ms.
The following are functions of Peptide mapping:
To confirm that a protein primary structure (amino acid sequence) including the N and C terminals.
1) To provide protein loci and the proportion of modified groups, such as glycosylation, acetylation, sulphates
a00nd phosphorylation.
2) To provide qualitative and quantitative information on protein degradation products
3) To get information on protein oxidation and deamination directly.
According to these useful features above, peptide mapping has already gained in popularity in biological
field. After getting numerous data from peptide mapping, the next step is deeper analysis.
A sample graph of peptide mapping
Peptide Mapping Analysis
Based on graphs of peptide mapping and also peptide mass mapping, scientists can get a lot of useful
information. According to the size of molecular weight of proteins, peptides and amino acid composition
characteristics, the use of strong specificity of proteolytic enzyme [is commonly endopeptidase] on the special
peptide chain site will peptide fragment into smaller fragments, through the separation of a certain form
characteristic fingerprint detection methods. eolytic enzyme [is commonly endopeptidase] on the special
peptide chain site will peptide fragment into smaller fragments, through th
The picture below shows the steps of peptide mapping method:
