Peptide mapping
Peptide mapping, the principal technique for confirming a protein’s primary
structure (amino acid sequence), can be used in drug discovery and
throughout the manufacturing process. It is one of the most important
techniques in comparability studies of biologics and a key step in the
characterization of biopharmaceutical proteins. For recombinant protein drugs,
such as monoclonal antibodies (mAbs) and antibody drug conjugates (ADCs),
peptide mapping can be used for identification, primary structural
characterization, and quality assurance/quality control (QA/QC). Some global
regulators, like the US Food and Drug Administration (US FDA) and the
European Medicines Agency (EMA), have developed guidelines to ask for
providing peptide mapping to confirm the amino acid sequence of drug
substance proteins.
Peptide Mapping Service at Creative Proteomics
At Creative Proteomics, we have developed a liquid chromatography with
tandem mass spectrometry (LC-MS/MS)-based peptide mapping platform for
confirmation of protein primary structure. And we can provide the peptide
mapping service to analyze the sequence of biopharmaceuticals and confirm
complete expression of recombinant protein in compliance with ICH Q6B.
Figure 1. Workflow of Peptide Mapping Service at Creative Proteomics
As peptide mapping service compares the peptide masses with protein
databases, only proteins from the database can be identified using this method.
Proteins, whose sequence is not found in a protein database, such as
antibodies or novel proteins, can be deduced by our service of de-novo
sequencing. In addition, we can provide detailed reports, including:
Experiment procedures
Parameters of liquid chromatography and mass spectrometer
MS raw data files
Bioinformatics analysis
Peptide mapping results
Our Advantages
Various types of proteases used to achieve coverage of 100%, such as trypsin,
chymotrypsin, Asp-N, Glu-C, Lys-C, and Lys-N.
