1. Justify the following statements:
a. A scanning electron microscope is used to examine the cell surface.
b. Wavelength of the illumination light plays an important role in microscopy.
c. FRET is an important technique used to study protein protein interactions.
d. The Fc portion of the antibody plays an important role in the immune based
assays.
e. Heavy metals are used as stains in TEM.
f. It is important to convert the element into a gaseous ion to determine its mass
using mass spectroscopy.
g. Fluorescence microscope has two monochromators.
h. Pull down assays are not usually used to understand Transient protein–protein
interactions
i. Bait and prey are used in yeast 2 hybrid systems.
j. PVDF is superior to nitrocellulose in blotting.
k. Plasmids and oligonucleotides are used for transfection
l. Formalin and paraffin are usually used to preserve the morphology of the cell.
m. Nucleic acids could be fixed to a nylon membrane.
n. Tritium is commonly used for autoradiography.
o. Biology does not use alpha particle emitting radioisotopes for studies.
p. EMSA and footprinting methods are in vitro methods to study protein DNA
interactions.
q. Nucleic acids may be transfected in the form of a plasmid or as oligonucleotides.
r. Tritium is an ideal isotope for use in high resolution autoradiography applications.
s. electron microscopy is performed under high voltage and vacuum.
t. protein microarray preferred over DNA microarray to study gene expression
pattern
2. Define the following:
a. Fluorophore
b. High throughput analysis
c. Immunoprecipitation
d. Transfection
e. Quantum yield
f. Transcriptome
g. Isoelectric pH
h. Reporter assay
i. Immunofluorescence
j. Proteomics
3. Differentiate between the following:
a. FACS and TUNEL assay
b. Scanning and transmission electron microscopy
