Yeast Expression
Yeast is the simplest eukaryotic expression system. It sustains post-translational
modification and proper folding of eukaryotic proteins, while still allows easy
culture and scale-up production at relatively low cost. The two most commonly
used yeast species are Saccharomyces cerevisiae and Pichia pastoris.
Features
Disulfide-bonded protein expression
Glycosylated protein expression
Intracellular/Secreted expression
Large-scale eukaryotic protein production at low cost
P.pastoris is the most commonly used yeast species for recombinant protein
expression. Culture of P.pastoris is simple and inexpensive. It grows fast and can
reach high cell density, which supports high yield and large-scale production of
eukaryotic proteins. It is also a recommended system for glycosylated or
disulfide-bonded proteins. Profacgen designed expression vectors with strong
promoters that support either secretory or intracellular production of your target
proteins.
Pichia pastors is a species of methylotrophic yeast.
They have a high growth rate and are able to grow on a simple, inexpensive medium.
