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Basic Concepts of Chromatography Quiz Chromatography TLC
Typology: Exercises
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S.No MCQ Basic Concepts of Chromatography 1) Chromatography is a physical method that is used to separate and analyse
a) Simple mixtures b) Complex mixtures c) Viscous mixtures d) Metals Answer: b Explanation: Chromatography is a physical method that is used to separate complex mixtures. The mixture of different components is flushed through the system at different rates. 2) In which type of chromatography, the stationary phase held in a narrow tube and the mobile phase is forced through it under pressure? a) Column chromatography b) Planar chromatography c) Liquid chromatography d) Gas chromatography Answer: a Explanation: In Column chromatography, the stationary phase held in a narrow tube and the mobile phase is forced through it under pressure. It is carried out in a long glass column having a stop-cock near the bottom. 3) In chromatography, the stationary phase can be ___________ supported on a solid. a) a) Solid or liquid b) Liquid or gas c) Solid only d) Liquid only Answer: a Explanation: In chromatography, there are two phases namely, stationary phase and mobile phase. The stationary phase can be solid or a liquid supported on a solid. 4) In chromatography, which of the following can the mobile phase be made of? a) Solid or liquid b) Liquid or gas c) Gas only d) Liquid only Answer: b Explanation: In chromatography, the mobile phase can be composed of liquid or gas. It cannot be a solid material. 5) Which of the following cannot be used as an adsorbent in Column adsorption chromatography?
a) Magnesium oxide b) Silica gel c) Activated alumina d) Potassium permanganate Answer: d Explanation: The given options are all examples of adsorbents in Column adsorption except potassium permanganate. Some other adsorbents are starch and chromatographic purified siliceous earth. 6) Which of the following types of chromatography involves the separation of substances in a mixture over a 0.2mm thick layer of an adsorbent? a) Gas liquid b) Column c) Thin layer d) Paper Answer: c Explanation: Thin layer chromatography involves the separation of substances of a mixture over a 0.2mm thick layer (thin layer) of an adsorbent. The adsorbent can be silica gel or alumina. 7) Chromatography cannot be used to purify volatile substances. a) True b) False Answer: b Explanation: Chromatography can be used to purify volatile substances if the carrier fluid, operating conditions and right adsorbent material are employed. 8) In Column chromatography, the stationary phase is made of _________ and the mobile phase is made of _________ a) Solid, liquid b) Liquid, liquid c) Liquid, gas d) Solid, gas Answer: a Explanation: In Column chromatography, the stationary phase is made of solid and the mobile phase is made of liquid. It is carried out in a long glass column which has a stop-cock near the bottom.
15) Which of the following types of chromatography involves the process, where the mobile phase moves through the stationary phase by the influence of gravity or capillary action? a) Column Chromatography b) High Pressure Liquid Chromatography c) Gas Chromatography d) Planar Chromatography Answer: d Explanation: In Planar Chromatography, the stationary phase is supported on a flat plate of paper. The mobile phase moves by the influence of gravity or capillary action.