bio 213 lab practical, Quizzes of Biology

Lab practical for bio 213 gmu 5 pages

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2025/2026

Uploaded on 12/04/2025

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BIOL 213. Practice Exam.
You should be familiar with ALL the techniques used in the lab. Remember that your final will be
mostly practical. This means you will be using the techniques/instruments to measure/observe
different metrics.
When studying for this exam, strive to understand the purpose/application of each lab instead of simply
memorizing the answers. Key study questions are:
oWhat are we studying in this lab? Why? What do we want to accomplish? Ex. To know
the concentration of protein in my solution.
oWhat are the variables involved? What is the metric used?
oWhat are the techniques involved? Why are we using this technique?
oWhat is your positive and negative control in each experiment? What does each of them
contain?
oWhat are the key definitions?
1. Using the diagram of the electromagnetic spectrum determine the color of the pigments in the
following graph. Why there is no absorption at ~ 525nm?
2. If you want to transfer 7 ml of water into a test tube. What pipette would you use? And for
15ml? Check the use of pipettes to transfer different volumes.
3. A sample is in focus at high dry under the microscope. What is the total magnification? Check
the use of the microscope, how to operate the instrument to high dry.
This study source was downloaded by 100000897617107 from CourseHero.com on 11-26-2025 22:41:45 GMT -06:00
https://www.coursehero.com/file/241525859/Practice-Final-Exam-RocioCornerodocx/
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BIOL 213. Practice Exam. You should be familiar with ALL the techniques used in the lab. Remember that your final will be mostly practical. This means you will be using the techniques/instruments to measure/observe different metrics. When studying for this exam, strive to understand the purpose/application of each lab instead of simply memorizing the answers. Key study questions are: o What are we studying in this lab? Why? What do we want to accomplish? Ex. To know the concentration of protein in my solution. o What are the variables involved? What is the metric used? o What are the techniques involved? Why are we using this technique? o What is your positive and negative control in each experiment? What does each of them contain? o What are the key definitions?

  1. Using the diagram of the electromagnetic spectrum determine the color of the pigments in the following graph. Why there is no absorption at ~ 525nm?
    1. If you want to transfer 7 ml of water into a test tube. What pipette would you use? And for 15ml? Check the use of pipettes to transfer different volumes.
    2. A sample is in focus at high dry under the microscope. What is the total magnification? Check the use of the microscope, how to operate the instrument to high dry. This study source was downloaded by 100000897617107 from CourseHero.com on 11-26-2025 22:41:45 GMT -06:
  1. Identify the stage of mitosis in the following pictures.
    1. Identify the following parts of the labeled microscope
    2. What is the volume it is set to transfer (include units)? This study source was downloaded by 100000897617107 from CourseHero.com on 11-26-2025 22:41:45 GMT -06:
  1. How would you prepare 25.00 mL of 0.1210 M CH3COOH solution from a 0.3003 M CH3COOH solution? Using 10.07 ml of 0.1210 M CH3COOH
  2. How many micrograms (g) are there in 2 grams? Use correct scientific notation.
  3. Calculate the concentration of DNA in a sample with ABS 260 = 0.77 and ABS 280 = 0.44 (hint— 50ug/ml of DNA has an absorbance of 1 at 260nm). 0.0385 mg/ml B. Is your sample pure? yes C. What is the concentration of protein in your sample if 100ug/ml of protein has an absorbance of 1? 44ug/ml
  4. Observe the DNA pattern of the crime scene, who is guilty (if anyone) of the crime? 14. Using the following values, construct a calibration curve. What is the equation? y = 0.0043x + 0.003. R² = 0.9893 (This equation does not include reading at concentration 0) B. What is the concentration of protein in an unknown solution with A (595nm) = 0.2? Use both the equation and the graph. Are these values similar? From the equation: 45.81ug/ml. Protein concentration (ug/ml) OD reading at 595nm 20 0. 40 0. 60 0. 80 0. 100 0. This study source was downloaded by 100000897617107 from CourseHero.com on 11-26-2025 22:41:45 GMT -06:

This study source was downloaded by 100000897617107 from CourseHero.com on 11-26-2025 22:41:45 GMT -06: Powered by TCPDF (www.tcpdf.org) https://www.coursehero.com/file/241525859/Practice-Final-Exam-RocioCornerodocx/