Download Biology 213 Lab Practical | BIOL 213 - Cell Structure and Function and more Quizzes Biology in PDF only on Docsity!
What does Lugol's Iodine detect the presence
of? and what color does it turn it?
Detects STARCH-If starch is present, it should be stained a bluish black color TERM 2
What does the Chloroplast consist of?
DEFINITION 2 -Outer Membrane-Inner Membrane-Space called the Stroma- Internalized membrane system made up of THYLAKOID DISKS-CLOROPLASTS ARE ATTACHED TO THE CYTOSKELETON OF THE CELL AND CAN MOVE AROUND INTERIOR SPACE TERM 3
What is Cytoplasmic Streaming? The plant
used to identify this streaming under
microscope is?
DEFINITION 3 -The continuous, orderly movement of chloroplasts in the cytoplasm.-Elodea is aquatic plant that has the thinnest leaves TERM 4
Where is Chlorophyll located?
DEFINITION 4 -Within the THYLOAKOID MEMBRANES of chloroplasts TERM 5
Chlorophyll a/b are green pigments that
absorb wavelengths of light two colors?
DEFINITION 5 -In the BLUE and RED regions of light Green light is reflected from the plant
What are carotenoids? What are
Anthocyanins?
-They are accessory pigments that may appear yellow, orange, red or brown. They absorb wavelenghts of light that the chlorophylls cannot absorb.-Anthocyanins are water- soluble and they do not absorb light for the photosynthetic process TERM 7
What device can you use to determine
wavelenghts of light absorbed by plants?
DEFINITION 7 A Spectrophotometer-Absorption spectrum is a graph that plots absorbance versus wavelength of light. TERM 8
At what to wavelenghts do Chlorophyll a/b
absorb most?
DEFINITION 8 Chlorophyll a absorbs lower at first (425 nm) dotted lineChlorophyll b absorbs HIGHEST OF ALL at first (475 nm) solid lineChlorophyll a absorbs higher second at (700 nm)Chlorophyll b absorbs LOWEST OF ALL second at (675 nm)Light absorbing pighment proteins have charcteristic wavelenghts at which their absorbance is optimal or maximal which is called (ABSORBANCE MAXIMA) (~475 nm and ~675 nm)- High absorbance bc wavelenghts have most starch or %amylose TERM 9
All of the photosynthetic pigments can be
extracted from chloroplasts and separated
by?
DEFINITION 9 Paper CHROMATOGRAPHY (but we did NOT use this method in lab we skipped it) TERM 10
Cytoplasmic Streaming (what magnification?)
DEFINITION 10 Cytoplasmic streaming is the directed flow of cytosol and organelles around large fungal and plant cells.-Observe under scanning objective first (10x), then increase magnification. Under what total magnification were you able to see cytoplasmic streaming? -usually high power because you want a zoom close up
