Download Bio17-module3-exam.docs.compatibality mode and more Exams Biology in PDF only on Docsity!
Questions and Answers
Bio17-module3-
exam.docs.compatibalit
y mode
- A nanometer is defined as: A. 10
B. 10 - C. - D. 10 - C. A nanometer is defined as one-billionth of a meter.
- True or False: A nanometeris longer than a micrometer. False. A nanometer is 1,000 times smaller than a micrometer.
- Resolution and contrast are two critical factors that influence your ability to see an object. Explain each. BIOD 171 Portage Learning/Module 3 Exam Questions and
Questions and Answers
Resolution refers to the distance between two objects at which the objects still can be seen as separate. Poor or low resolution means two (or more) objects may appear as one.
- Contrast is the difference in light absorbance between two objects. Poor contrast gives a high background and makes the visualization of multiple objects difficult. For instance, trying to identify 2 dark colored objects at night (low light = low contrast) versus the same 2 objects in the middle of a sunny afternoon (bright light against 2 dark objects = high contrast).
- Assuming a constant (non-adjustable) light source power, identify the part of the microscope you would adjust to limit the amount of light entering the microscope. Select all that apply. A. Objective B. Condenser C. Iris diaphragm BIOD 171 Portage Learning/Module 3 Exam Questions and
Questions and Answers
C. Virus with a diameter of 0.2 μm D. Skin cell with diameter of 1500 μm A and C. The unaided eye can, on average, clearly resolve objects > 100 μm BIOD 171 Portage Learning/Module 3 Exam Questions and
Questions and Answers
- Label the following unmarked microscope components (numbered arrows) by matching it with the components provided (letters). BIOD 171 Portage Learning/Module 3 Exam Questions and
Questions and Answers
4G
5A
6H
1. This type of microscope utilizes ultraviolet (UV) light to illuminate stained objects.vFluorescence 2. This type of microscope uses a specialized condenser and objective to amplify the slight differences between cells and background.vPhase- Contrast 3. This type of microscope enhances contrast between specimen and background but does not permit the visualization of intracellular structures. Dark Field 4. This type of microscope uses neither halogen nor UV light sources but rather lasers to illuminate stained cells in high resolution. Confocal
- Identify what type of electron microscope was used to BIOD 171 Portage Learning/Module 3 Exam Questions and
Questions and Answers
capture the following image and explain your choice. The above image is captured via a Transmission Electron Microscope (TEM). Even at 20nm resolution (inset image), subcellular substructures are still visible. The image lacks the outside ‘shell’ only appearance of SEM.
- Gram-Positive cells appear in color due to a peptidoglycan layer in the cell wall. BIOD 171 Portage Learning/Module 3 Exam Questions and
Questions and Answers
2. You suspect a patient may have TB. Once a sample has been obtained it is sent off to the lab for an acid-fast stain. If the patient were infected with TB, describe what you would expect to see on the stained slide. You would expect to see red cells (TB+) on a blue background (TB negative). 3. True or False: If a patient is suspected of having malaria, a Giemsa stain would be an appropriate differential test to perform. True. Giemsa stains are often used in the clinical setting to aid in the diagnosis of blood parasites. BIOD 171 Portage Learning/Module 3 Exam Questions and
