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Questions and answers related to Module 3 of the BIOD 171 course offered by Portage Learning. The questions cover topics such as nanometers, resolution, contrast, microscope parts, staining, and types of microscopes. The answers provide explanations and examples to help students understand the concepts. The document also includes an image captured via a Transmission Electron Microscope and a section on Gram-Positive and Gram-Negative cells.
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C.10- D. 10 - C. A nanometer is defined as one-billionth of a meter.
the middle of a sunny afternoon (bright light against 2 dark objects = high contrast).
B. Fine Adjustment Knob C. Iris Diaphragm D. Neck E. Condenser Lens F. Eyepiece G. Objective H. Base I. Coaxial Controls 1F 2D 3B 4G 5A BIOD 171 Portage Learning/Module 3 Exam Questions and
The above image is captured via a Transmission Electron Microscope (TEM). Even at 20nm resolution (inset image), subcellular substructures are still visible. The image lacks the outside ‘shell’ only appearance of SEM.
peptidoglycan layer in the cell wall. Pink; Thin
1. True or False: Following the decolorization step of the Gram stain, Gram-Negative bacteria will appear colorless. True. Even together, the LPS and thin peptidoglycan layer are unable to retain the crystal violet dye during decolorization. 2. Name one substance capable of chemically fixing cells to a slide. Any of the following are true: Paraformaldehyde, ethanol or methanol. 1. You want to observe the size and shape of a cell. What is the easiest staining technique that you could perform? Name at least one dye you would use during this process. Simple stain. You could use any of the following: methylene blue, crystal violet, safranin or fuschin. BIOD 171 Portage Learning/Module 3 Exam Questions and