Bio17-module3-exam.docs.compatibality mode, Exams of Biology

Questions and answers related to Module 3 of the BIOD 171 course offered by Portage Learning. The questions cover topics such as nanometers, resolution, contrast, microscope parts, staining, and types of microscopes. The answers provide explanations and examples to help students understand the concepts. The document also includes an image captured via a Transmission Electron Microscope and a section on Gram-Positive and Gram-Negative cells.

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2022/2023

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BIOD 171 Portage Learning/Module 3 Exam
Questions and Answers
Bio17-module3-
exam.docs.compatibality mode
1. A nanometer is defined as:
A. 10-3
B. 10-6
C.10-9
D. 10
-12
C. A nanometer is defined as one-billionth of a meter.
2. True or False: A nanometeris longer than a micrometer. False. A
nanometer is 1,000 times smaller than a micrometer.
1. Resolution and contrast are two critical factors that influence
your ability to see an object. Explain each. Resolution refers to the
distance between two objects at which the objects still can be seen as
separate. Poor or low resolution means two (or more) objects may
appear as one.
*Contrast is the difference in light absorbance between two objects.
Poor contrast gives a high background and makes the visualization of
multiple objects difficult. For instance, trying to identify 2 dark colored
objects at night (low light = low contrast) versus the same 2 objects in
BIOD 171 Portage Learning/Module 3 Exam Questions and
Answers
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Bio17-module3-

exam.docs.compatibality mode

  1. A nanometer is defined as: A. 10 - B. 10

C.10- D. 10 - C. A nanometer is defined as one-billionth of a meter.

  1. True or False: A nanometeris longer than a micrometer. False. A nanometer is 1,000 times smaller than a micrometer.
  2. Resolution and contrast are two critical factors that influence your ability to see an object. Explain each. Resolution refers to the distance between two objects at which the objects still can be seen as separate. Poor or low resolution means two (or more) objects may appear as one.
  • Contrast is the difference in light absorbance between two objects. Poor contrast gives a high background and makes the visualization of multiple objects difficult. For instance, trying to identify 2 dark colored objects at night (low light = low contrast) versus the same 2 objects in BIOD 171 Portage Learning/Module 3 Exam Questions and

Questions and Answers

the middle of a sunny afternoon (bright light against 2 dark objects = high contrast).

  1. Assuming a constant (non-adjustable) light source power, identify the part of the microscope you would adjust to limit the amount of light entering the microscope. Select all that apply. A. Objective B. Condenser C. Iris diaphragm D. Eye piece C. The iris controls the amount of light that passes through the sample and into the objective lens. Thus, it can be adjusted (opened or closed) to alter the amount of light. 2. What is the total magnification (relative to your eye) of a sample imaged with a 60x objective and a 10x eyepiece? Show your math. 60 x 10 = 600x magnification 1. True or False: Staining is often required to image a cell that is BIOD 171 Portage Learning/Module 3 Exam Questions and

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  1. Label the following unmarked microscope components (numbered arrows) by matching it with the components provided (letters). A. Stage BIOD 171 Portage Learning/Module 3 Exam Questions and

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B. Fine Adjustment Knob C. Iris Diaphragm D. Neck E. Condenser Lens F. Eyepiece G. Objective H. Base I. Coaxial Controls 1F 2D 3B 4G 5A BIOD 171 Portage Learning/Module 3 Exam Questions and

Questions and Answers

The above image is captured via a Transmission Electron Microscope (TEM). Even at 20nm resolution (inset image), subcellular substructures are still visible. The image lacks the outside ‘shell’ only appearance of SEM.

  1. Gram-Positive cells appear in color due to a peptidoglycan layer in the cell wall. Purple; Thick
  2. Gram-Negative cells appear in color due to a BIOD 171 Portage Learning/Module 3 Exam Questions and

Questions and Answers

peptidoglycan layer in the cell wall. Pink; Thin

1. True or False: Following the decolorization step of the Gram stain, Gram-Negative bacteria will appear colorless. True. Even together, the LPS and thin peptidoglycan layer are unable to retain the crystal violet dye during decolorization. 2. Name one substance capable of chemically fixing cells to a slide. Any of the following are true: Paraformaldehyde, ethanol or methanol. 1. You want to observe the size and shape of a cell. What is the easiest staining technique that you could perform? Name at least one dye you would use during this process. Simple stain. You could use any of the following: methylene blue, crystal violet, safranin or fuschin. BIOD 171 Portage Learning/Module 3 Exam Questions and