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Biochemistry Lab Notebook Name: Ishan ‘ Y Date: 4-aZ - Lb Experiment #: Title: Isolate of an Eng qm from Py heort Purpose: fe pousrpoore Yrs lab us ee solte glutrmodeé- ogaloacetate While wolates Chat 2mggne ak ik te vrchvcuee. GOT Serdaes tase. ana jungrone Aloe Tee aie a ba lpr mahi oep3 , Meee cate ye ifs ii aad jcc pe beiry. ay Be to imtrachion AMIN LAE S / 2 Procedure Data/Results/Calculations Fat £: + Don ate. opens oi ey, a bberols » Diaee 100 me of nel plenal -telol tee toll K* 404 Vnlor “ A MinvrTe Leasare #5 mg of Hae buncied mek, while ebbing asiale a Bur 15 by into 250 mi eer pege ing place ‘eu 910°C, attr pestle JA of minucbey, nol Bo me ¥ Alpha Kelog Luctemare te Fry & Tad I Jpottndto water bath por ao munades ier omanat pout pote an see both ger ao a gael inoten & Gaotien L lh) nthe 9 T aml tig ‘ tons. afeth fee Fraston , incl affrw to SUH oats alhsog- Auce wasielé centrifuge and (ce bat Fiction a fa 35.2 mt Printed Name: shan: ol Signature: Date: 4|s0|2 Biochemistry Lab Notebook . Procedure Y ee mk huffer un best pee ‘lol B00 yraero lu FES of deena lofted NADH gen adlot Ge tated alphe bak pguteret® ih e inuAkS , 4 min porte dhon adol f muculete spectre ~200 me Whine ust ofhee p+ wtp cuvthe Bun for an _ — - +3 go prnptone mf” 2 yheht? P Su add model dd Fd a nantes f TOM Uinmterate @ GOT - ais te ‘ed /wicrolter H dais mn spe oA innate +t: | Adel Rm of unseteon EF. de seven ming Hues, | Add) 20 eierolters OF ammonium quifate to cath tu!o%| Sit iw ‘Gee bath had QD minut eS dine place PicroceranisUqe- . Remove. suprenctont grom Bach thee Pe dissolve bug, addine burfer [Ren assey eae clube For Annet es Addl 52.8 me 7 ammonite Fe Dati ant gp supe rerant }, si AeL4S (> ploee AS “Tie Dote Bnols -ok the tobe allow +o sit Yor: 24 ws. AB sich hp SEGUE ned pa aAz Irihal Absorbance ° Ar aie me tA “ies yk PA Data/Results/Calculations Pt 3: ae Inca ak alesov oan Ce @ 90:02 1 23% ‘be . " svery In-rand ack MOUNT 934 © 04:09 min _ T1156 Onk (f= 45.2me Aerwihy- Dawe 2 + Bmt) weldtutes Awe bp.22 m Hom! ol mt ce ye 0,942 -0.164 ‘ \ ee —_—_ - , : Frain b.22 Hem Bet e = 15.2 mmad|minfm& QoT 0.792 madlmnlac GOH _ _ Printed Name: /5 HProtewr petiek @ bettem large, eplor , progreasad cer eg 1S0me — Fre 7 35-% Tube Volume of, Ammonieea | _ Oo 2 0.40 50 Z| At 55 ff it Q-.w’o (7) 5 0.4 65 b 0.94 Yo q ; | 4.20 \ 45 
