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BIOC 440 Lecture #2: pH and Buffers in Biochemistry - Prof. Rachel Klevit, Study notes of Biochemistry

University of Washington (UW) - SeattleBiochemistry
Prof. Rachel Klevit

A lecture note from bioc 440, focusing on the importance of ph in biochemistry and how buffers work. It covers the principles of buffers, practical considerations, and their role in biology. The document also includes assigned reading, review and test questions, and homework problem set. It discusses the carbonic acid-bicarbonate system as an in vivo buffering system and explains the henderson-hasselbalch equation.

Typology: Study notes

Pre 2010

Uploaded on 03/18/2009

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1
BIOC 440 Lecture #2 1
“pH: Why should we care?”
I. Biochemistry is pH-dependent.
II. How buffers work
A. General principles
B. Practical considerations
C. Buffers in biology.
•ASSIGNED READIN G: Chap. 2: Section 2.2 & 2.3
• For a more thorough description of blood buffering, see Web links
listed on BIOC440 Schedule page.
• REVIEW & TEST yo urself online (see We b link listed on BIO C440
Schedule page.)
• HOMEWORK PROBLEM SET #1
2
Fig. 2-21
Biological Systems & pH
• Biochemical processes &
reactions are pH-dependent.
• Cells & organisms maintain
constant specific pH value that is
optimal for function.
Example: blood pH = 7.4
IF blood pH < 6.8 or > 7.8...
3
Buffers in Biochemistry:
in vivo & in vitro
Gen’l Def’n:
Practical Def’n:
Fig. 2-15
4
How Buffers Work
Henderson-Hasselbalch Eq’n…
so much info in so little time!
pf3
pf4

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Download BIOC 440 Lecture #2: pH and Buffers in Biochemistry - Prof. Rachel Klevit and more Study notes Biochemistry in PDF only on Docsity!

BIOC 440 Lecture #2 1

“pH: Why should we care?”

I. Biochemistry is pH-dependent.

II. How buffers work

A. General principles

B. Practical considerations

C. Buffers in biology.

  • ASSIGNED READING: Chap. 2: Section 2.2 & 2.
  • For a more thorough description of blood buffering, see Web links

listed on BIOC440 Schedule page.

  • REVIEW & TEST yourself online (see Web link listed on BIOC

Schedule page.)

• HOMEWORK PROBLEM SET

2

Fig. 2-

Biological Systems & pH

  • Biochemical processes &

reactions are pH-dependent.

  • Cells & organisms maintain

constant specific pH value that is

optimal for function.

Example: blood pH = 7.

IF blood pH < 6.8 or > 7.8...

3

Buffers in Biochemistry:

in vivo & in vitro

Gen’l Def’n:

Practical Def’n:

Fig. 2-15 (^4)

How Buffers Work

Henderson-Hasselbalch Eq’n…

so much info in so little time!

5

How Buffers Work: 2 Equilibria Involved.

Fig. 2-

HAc H

  • Ac

H

  • OH

H 2

O

NET =

6

Shape of titration curve is same for all weak acids

Fig. 2-

7

The right buffer for the job…

  • maximum buffering capacity
  • buffering range:
    • buffer concentration & capacity

How Buffers Work

8

Biology’s buffers

Physiological pH = 7.4 (blood)

In vivo buffering systems:

Carbonic acid-Bicarbonate system: multiple equilibria

H

3

O

  • HCO 3

H 2

CO

3

+ H

2

O 2H

2

O + CO

2(d)

CO

2(g)

K

1

K

3

K

2

Fig. 2-16^ BIOC 440 Lecture #2^13

REVIEW: How to calculate an EQUIVALENT for a

buffer?

General form of NET Rx:

HA + OH

A

  • H 2

O