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Olevel 5090 class notes BIOTECHNOLOGY
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It is the exploitation of living organisms for the benefit of human beings. Biotechnology has 3 types: Farming using tissue culture technique Fermentation of alcohol and yogurt Pickling Mainly bacteria is used in the process of genetic engineering because they can be cultured to make colonies and grow rapidly. It is also used because of the presence of plasmids. The most used tool in Biotechnology is Yeast is unicellular. TISSUE CULTURE TECHNIQUE: In this process, the Meristematic tissue of a plant or also called the Cambium tissue of the plant is transferred into an artificial environment and given the main requirements: Water Nutrients Minerals Suitable temperature Here they continue to grow and function and soon a seedus emerges and then after some time it is planted in the real environment where it grows into a proper plant. FERMENTATION: It is the process in which organisms convert starch or sugar into alcohol by anaerobically releasing energy. FERMENTATION OF BREAD: Firstly Maize is treated by Amylase and converted to starch. Then starch is treated with Amylase to convert it into Maltose. Then Maltase converts Maltose to Glucose. Then Yeast is added in the Glucose which respires anaerobically producing ethanol and CO 2 (trapped in bubbles) that help the dough to rise. In the process of baking the yeast is killed and the ethanol evaporates
Glucose (yeast respires anaerobically) Ethanol + CO 2 Gluten is the component that makes the dough stretchy. Fermenters can be used to produce useful products by using bacteria and fungi. The Conditions that need to be controlled are: Temperature pH Oxygen Nutrient supply and waste products. USE OF ENZYMES IN BIOLOGICAL WASHING DETERGENTS: Enzymes are present in Biological detergents or washing powders in the form of packings, the size of micro-granules. They are coated because the enzymes can be denatured easily if exposed to extreme high or low temperature. The detergent needs to be wet because it needs a suitable condition to be activated. Biological detergents contain the enzymes: Protease Protein Amylase starch Lipase Lipids USE OF PECTINASE IN JUICES: The enzyme pectinase is used to treat fresh fruit as it breaks down pectin in the fruit. This helps in the improvement of the quality of extracted juice. The enzyme enhances the process yield and accelerates the clarification of extracted juice. USE OF LACTASE IN LACTASE-FREE MILK: Lactase, the enzyme added to lactose-free milk, breaks lactose down into two simple sugars: glucose and galactose. Because your taste buds perceive these simple sugars as sweeter than complex sugars, the final lactose-free product has a sweeter flavor than regular milk. Lactose (lactase) Glucose + Galactose