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Chapter - 4 Biotechne nology < Phinctples And hho cesses y? # Bio dechna lo £ techmque * ab kul tev ng okgensm . v u * Eanepean Fedexation of Biatechnalage .— M isthe tnd eg mal i & of natusal aseiencXO and living omganicsm =a cell a pants ox meleculdu. “analogue for _vasicus fsoducls and Surwices— | Tyges cf Biotechnolo 9 Classical Bic etc id Fosmatien of ant t - tike ctitsic acidlY 2 abechot - feumentotien , cus = i “Hodewn Biotechnology > a S$ is _selated dit, enehie Moanifuloation | Se lnagh PNA dechro lo | Inenedic _engini eoni ay -lecattic ineeaing — manipulation sf genetic yn ety material d Chemical engineeni ng - be file envisiomment for mi CHobes a 4 J 4 Reastsiction Enzyme ee ‘ Resisviction the mulhplication sf yitus- Bactenia aftectes by Vimus ' iy ‘ col Bact aio Page. ihe ae a a Bees. . =a des‘as |, ee) is nol int eg Fea.ted _ with backesuia Bea’ then it js ™ 2 not poss in next a? a -) + Vittal Bn is tntegrated with boctesal DwA do fas next | genenation: F + Postyichion Pig aluays pets on fabindso mic | : a al ~Bequence stl -fectaicten one =e _Kecegnise vistal BNA and | a ba) the. Bue - | 2nzyme ~ and me thy tore - > Worked Y “by Y the _ hele methy dase b acletia medi fires ‘ ¢ a rere a, sequence this is © Catied — uostereha; Meat; Cahir a System 48 Rectuicti on ae do. be. di’s covered: ie 2 ¥ oe Dee His a ie a Roman ye ox rsolahon a ee Z einyh og *_gahrnis, tak by a »~_ ao @& Bo © io if _Hamita. — Baccilus amyle lique faciens NS btn A Thee aslicky CCTA bin bee Tools of biodechnology ie t) Restsiction enzyme N; Dy. PNA Poly mnesase. J Ona NWyase. \P ~) Clontag” veetos \ ‘ le Conpelent hoard: W Le i Aa Reastur chen Ona, : | » Alkaline. - Fook —_ a6 Bacleual - sAllaaine “phosphate, CofP atkaline . phosphate. a -Plespade a Stondl o> Phusphade memove coe Phi poe Poo | igen Si ca ra ee es ate : Se enzymes belongs to a langes Class of ie mes Callél __nucleo4es- These Owe tuo types norucdease i- exonuclease kemove —nucleotideY eS fn te endt of ANA - : 7 Pp. eke hs PE “> make. Cts at os eerfic _foss: tions _ : )-0 © © © 6 eae a eee) a a a ba ga ea a maeanneeve et 2i2.2a.d within te DnA- Each sestoiction endonuclease Kero nite a upecr fi. Palincltomic nucleotrde WBE QuENLe in OA The cutti "9. 4, Sw neatact ion endonuclease SMesults f Hag mente of QNA- “these Fsagmer Can . be 0 EN ated by Fechnigue kin wan * a6 aty oO Plan gs re ; as ine ef é lee (Oe ce i "emt cea. veclox =- Shee me Aiscrvouods by ‘HDs aed ladlesbery geek teak ; g Terre sep coding. Lo ae ae Pepe so APY) AS a) tes ar. PP. eal Osi site -__apecific Qa osequence—_nesfanaible ig fe —iitiaig of weplication- - oelectoble ue “mankey. = soe Thons Sxmant Nion= 1- spranafee- ae ___._mant onhibie poate _ gearutont : Gs! faaying e935 Zing ae iH ecogation ate # lagmi id bas ed !- “a " : : ) tiga fase colo uriny 9 BMxn « ames wey 4 catt famia © ie Sriid —_ Animales —+ Retenovinus te Sidiaad P a e — Revenwse deans cuiptase a — me « flanis — Agro bactetulum dumifaci eng e a Mi r a Ti ae ( Tumex inducing Plato noe ll <<: Cause _ _CXown Ss a -_ J = - ae e rr a : = ea aa A al eae : _ oom 4 Turns acy gene e A EE Oa id (1) cansfon.galesmid Y- eee tine gs > flesm 2 ee a ms ook Se ee. —— He eS = Peo. a a a - ee a ee ee eS goose fe aa aa ees 3 i e = er oH Ircsoutional inactivation; — aHeunative oselectane o _roukexs hove been. dleveloped which ali Plow ent s —_secombinanks fsom non ~_ 4ecombinanks on tage o _tasis of — theix vai bp Puoduce Cola in puesy e ef _chitomogentC trate - “In this Q__He core y noont Dwi 18 ingesded within tis apie » ——of 00 enzyme f agalac toasidase - “this ¥ wes ul \ € , uf = !n _ inaetivation of ee for Bunthe sions of this -~ “Ar which sefesuvd do 4s $ ie insextional inactivation - : I Competent Moat i= 4 | 2 Bactlenial cel) - . __* Yeast. cell: i ee i - s QvA = bydngtiic. . . a aso it ae easily fas 7 — raemb @ ~ = we . Can ae “ Sf cial ds 4 ~~ + Toueat Got _celt_ eh _ Calcium tall ath, ve — —ashock S Se pe ara =. = = > es ee f ess. Y i Y i é ae bk = oS ) eee = = - Mi ote ingect® ae ine, _ Dis tovened. re — Da Boule: = — = WEE Pie ee and _ mi copifet = ene , a eee =—boogte 4— Ovum _, embryonic cell. leebiofasralion :— PHe to Plasen of cell exo aed _elechyic — ped ii) 1h) is) there On the. fo | ie field A Ha igment s ae J hucush there QnA Psa de ro NS Osi ‘the sep peated 2A ie ACEO. nein visualyse nA wots b). Tsolation- of the. sepesiaded foo ti ts +. aT “A ‘ foe menias cat be af tex. | the Bai ining of othi aliam bx mide / ete J Canemnoegen - C desited DyvA monts 1 ~ band of ane Cul a fel and extracted ie is Knawn PCR stands. fy - Enh__folymevase » -the___TAO_ polyrmexase (a heat stesiostar iss Dnd__seplication for _sealise en_ the Chemicals: ul the = POR res __ be tanist. ie at dice: Basi coll POR Cale > “othe _ Bevorap OMe move andex the elects. 8/2 Lay) aa way db, medrtum Bepenate d 2€ end chass rr elation BPsate out te Onp Faasn ce ification of gene usi fol umes _ocliscovened by Ho Hulls recprose Sell Pe. Reactien an Amexican in 1970: _ tasoloted pp orn __ the bacteria oguati Cus+ this bactetu a — Can demp exature upton 4S ¢ én a large a fp U use _ of U tC #n2yrsc} the: mus’ Aus) ve PPPEPDET H TPT tH Ht a ) 1 x Coat iv) A asmalt amount Qnyh tp ci ? wi traf template 5 " oe : = iti) the. fous Sipes of deony suibonuclia tees == Gras + A asingic = PCR guxn- involves 3 ce exature — d clepend ent wep : Md ) Penakuxation.: the oem ding uslulien #s heated roars at aie hd ai kk —? en hwsik the Syn Sy: PEETL A igri. herd — an Canvikl ome deubie wtrand’ DA iad single aubyand Cd = a d @mme ji) fnneline :- adding of Prine is Known as — anneline + L = 60 a 2m lee ii) Extension \ Pa ymexisatien i— in this aitepe. the wheng-- —— Sn fly ekake add nucleotide hr furmex * nas ae and athesise. a new Spf ian _—— This “Tisiep ra | ae . a i = Ipplicalien cf fee Orme 3) Detoction ee _fatho See 5} Detection of _ mut See tue notal re vaca : ke Cezamet ‘w) PHetection of 9 michedb es iN _awsail y) Used in fa.tonsic lab 5) Toansfox of dexised gene into _vechx - 6) Thansfe of yeokr fn fost cell TAARV9900808 . | Page Np. / | Dare [31/9 /ay) Questions (Ans us evs @eoee © Que-') Can list lo 1ecomb inant prodeins which ame ® used jin m ical Psactice ! 7 Pind. out whese they awe e used as therapeutics 7 7 p fns-i) Human ingulin— Diabetes » ii) Ruman wth ae Dwasfism cune , tii) Blond oe facto Ytn/Tx — Remophilia _ tv) THA (dissue Plasminogen octivalos) - Heast atlack fstroks v) (BHE ( platelet denived ee oo Btimulates wound : healin vi). Tatesfexon— Taeotment. ~ of Vinal in fection: d Vii) Tnteyli = Enhances __immune ection - he vaccine ~ freventien. of infectious clisease | hespes vaccine = puevention of infectious disease. A) nose I~ THeatment of “talc Si Gas om Que = 2) Fuom what you have leaunt , can you fell ashe they LL _bi ok QnA is bi 68 Bale _moleculax | Y essa How did — yous v as 2 me Ans,- _Enzumes ane — om than QwA ov they ase. proteins and _ “puoteing ave” mactomoterwet mide of amine oe ase. _bigges than een This_can OMoved hokesis whexe aac. Pe by Ha ly not ‘move but denatured DNA ill — ee distance - uot ein esis is egal by osmall Se: of VOnA Called Te 1e- 3) ® eakanychiccels ove estuictien endonuclrse = Alastify. one ang 2% | i Mms- No oukax Ovo otic Cells do nol fave megtsiction 9 endonuclease Y pocause Dwh motecules of hun axe _heavil meth lated: All stoatniction endonuchbase o= have béon idated fxom Vanious stbsain of bactestia : Que-4) Bosides bette aenation and mixin fHof ties what other advantages do etimned — tonic biosteacty have ov os er f loa kes Ans- AShoke f losks ane used ox re SLs mid the _ olesiked 2 mates ascote in the Labo -A Ree recall pce ‘/ desined Ketechno dd is doe ; using “hosteachys’._ ae _betten on. an mixing _ poopercties , the ‘biaxeachns have following ___advant 1) Small © yoeumesx _ Cian ode. Pesioccal 4 -vitadgnnn —_fatoon — “die sechs fox swamp li i) Tt fos a fram. Condsob _atystem , fA cants¥L csyshem “ond. ee eontsol. mane zi) Fock lities Waa Ses: i 5 en avout ie thoughout the ofa : = Que 5) Con ee and _ankwes. how the ec ep be on - ot On be pa db monitor dans fosmation a Vhowt call af paste Dna in adalition to a ____ polectable fra A Se poste enayte Car. be. used to ut - Fesfoamed celts ty sacking down the — activity of its 0 = Mepondiing gene (Me ceptos— | Page Np 1 Ee Date | (a ii ™_~ .: ee yen tem, a dempestadu xe. Content © — r{ NF duster, PH Cay cays ana Bamplin: 05}; 4 - di ‘a d p pom 1333] Down wt eam psoceasing +- The fsoduct obtain ea a is subjected cb a eHiOR foc esses Colle chvely called — downsteastm _ PHocess ing befane ". hes pet er thastios ta finished. duct Cmamgy oe fox. masking - the doo — main (4AOCE3S Cag TS. axe © spemation and pasi freatien- The pxoduct is then 1h with Buitable od beh ttl T» pee ae buch psmulakens ae i | See adele ile Srails in cose of abuuga: T™ e-4:) Bui of dos cui bo tho. eae T». PR .-_ a ie _seaction Lin vGbro Sata fea Tio ite biological technique Ay matical PO solic DA _wifout using “lying "agin — Buch Jos 6 -Cob’ or east , > Theee TORE Wo & AM cea — [> Denotunation ' of desixes double ahand Qh pA — Bei, oo ead 5 Gm i: Anneli pi pM eH uo 940s Dn = iW Eddensil 3 phim Oe by “Tag OMA folymerage —— “isslated from. Thew mits aguati ats - om | = nlf ication. of | “cles: ued gene! gene Cloning - a Ho xe oul? ‘ut er) rN com a: Fr ike ofeh 4 geen , less ence. a cyclic — and dutomated - Trier? eS “Restoucd (en re me. is Q gMouy p n2ymes Ona ae in each’ havi at which it es, 5) ee Dyce poterin cell AT 4e8 tin it = asite * Recon asite i) They ane VY endonuclease POS, i). they ¥ freduce aki mat mtion. _ aite z Restart o en. eee there foHe. _a_ mechanism eriolved —vistol attack — and do _KK —. > Ce “4 Pl ibbl®! & weve! = ” bmoakdow a of fagal “ate tp_ on 34 a. Cais Du. m 2} fadrornaus f -seplicae. we iii) Not attached — < _fstones - and DNA 3 used to Cleave chnactexs tics bose Ova fexomn ent exi various 94 is and ends: Clea v oot oli Pexent oo tly Stine C)_ Chitinase 2- chi linase iso cane | bonds in Uchitin cell rade facilitate. —its_—cbenns fama Difpeuontiok _botween .— Restyict ton or | os Cut oe} VeGu ence nomad 0 gnitien fPabin rae exonuclease bot Bite and fuom Cach oher- OK e beds eved to be. ewia tb mesial: _semoval vital ah'en eee Suh W Kephicates und ex — nuclear amtyol-- Gi) Double awteancled , Cixculox 0 oe (isi) Associated — wrth histone puotei n 
