Investigating Biological Solutions with Paper & Thin Layer Chromatography, Schemes and Mind Maps of Biology

An overview of chromatography, a separation technique used to identify and analyze substances in mixtures. The focus is on biological solutions and the use of paper and thin layer chromatography. the principles of chromatography, the role of pigments in leaves, and detailed instructions for carrying out paper and thin layer chromatography experiments.

Typology: Schemes and Mind Maps

2021/2022

Uploaded on 05/19/2022

AdilH12345
AdilH12345 🇬🇧

8 documents

1 / 11

Toggle sidebar

This page cannot be seen from the preview

Don't miss anything!

bg1
Chromatography
Investigate biological solutions using paper
and thin layer chromatography.
C: I can describe the
process of thin layer
chromatography.
A: I can explain how I
would use chromatography
to analyse different
biological solutions.
B: I can explain why
different solutes move at
different speeds along a
THC plate
pf3
pf4
pf5
pf8
pf9
pfa

Partial preview of the text

Download Investigating Biological Solutions with Paper & Thin Layer Chromatography and more Schemes and Mind Maps Biology in PDF only on Docsity!

Chromatography

Investigate biological solutions using paper and thin layer chromatography. C: I can describe the process of thin layer chromatography. A: I can explain how I would use chromatography to analyse different biological solutions. B: I can explain why different solutes move at different speeds along a THC plate

Chromatography

  • (^) Separation of substances in a mixture.
  • (^) Identification of substances using Rf values.

Chromatography

  • (^) Separation technique that relies on the affinity of components of a mixture to:- - (^) A Mobile Phase - (^) A Stationary Phase
  • (^) All types of chromatography rely on this
    • (^) Paper chromatography
    • (^) thin layer chromatography,
    • (^) high performance liquid chromatography,
    • (^) gas chromatography

RP7: Pigments in Leaves

  • (^) Leaves contain multiple pigments each absorbing a different wavelength of light.
  • (^) Having more than one pigment will increase the range of light a leaf can absorb. In addition to photosynthetic pigments leaves will have other pigments with essential roles (U.V protection). Different species have a different composition of pigments.

Known Rf Values (retention factor)

Two Potential Methods:

  • (^) You are going to write your own method for this experiment as if you were completing it in two different ways:
  • (^) Paper Chromatography
  • (^) Thin Layer Chromatograph

Required Practical 7: Chromatography of

Photosynthetic Pigments

Two Potential Methods:

  • (^) Thin Layer Chromatography:
  1. Read page 35 and write a concise plan to carry out this method using Thin Layer Chromatography.