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Biotechnology II Exam 2010-2011: M.Sc. Degree (Biotechnology) - Section A, Exams of Biotechnology

Punjab Agricultural UniversityBiotechnology

Information about the spring examinations 2010-2011 for the m.sc. Degree in biotechnology, specifically for module bg502 - biotechnology ii. Exam code, modules, paper number, external and internal examiners, instructions, duration, department, and co-ordinator. Section a of the exam covers various topics such as determining the volume and impeller speed of a large-scale bioprocessor, assumptions of richard's sterilization method, and holding time calculations for achieving a certain risk of contamination. The document also includes a table with temperature and time data.

Typology: Exams

2011/2012

Uploaded on 11/30/2012

gurudev
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bg1
SPRING Examinations 2010 / 2011
Exam Code(s)
1MT1
Exam(s)
M.Sc. Degree (Biotechnology)
Module Code(s)
BG502
Module(s)
Biotechnology II
Paper No.
2
External Examiner(s)
Dr. John P. Quinn
Internal Examiner(s)
Professor Vincent O’Flaherty
*Dr. Aoife Boyd
Instructions
Answer 4 Questions in total.
TWO questions from Section A
&
TWO questions from Section B
Use a separate answer book for each Section
Please indicate clearly the numbers of the questions
answered on the first page of your Answer Book
Duration
3 Hours
No. of Pages
1
Department(s)
Microbiology
Co-ordinator
Dr. Aoife Boyd
Requirements None
Release to Library Yes √ No
PTO
pf3

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Download Biotechnology II Exam 2010-2011: M.Sc. Degree (Biotechnology) - Section A and more Exams Biotechnology in PDF only on Docsity!

SPRING Examinations 2010 / 2011

Exam Code(s) 1MT Exam(s) M.Sc. Degree (Biotechnology) Module Code(s) BG Module(s) Biotechnology II Paper No. 2 External Examiner(s) Dr. John P. Quinn Internal Examiner(s) Professor Vincent O’Flaherty *Dr. Aoife Boyd Instructions Answer 4 Questions in total. TWO questions from Section A & TWO questions from Section B Use a separate answer book for each Section Please indicate clearly the numbers of the questions answered on the first page of your Answer Book Duration (^) 3 Hours No. of Pages 1 Department(s) Microbiology Co-ordinator Dr. Aoife Boyd

Requirements None

Release to Library Yes √ No

PTO

**SECTION A

  1. Answer parts a, b, c AND d** A large-scale bioprocessor system has been designed based on the performance of a model system with a cylindrical geometry with the following characteristics: Volume: 16 L Impeller diameter: 80 mm Impeller speed: 800 rpm Fluid density: 1420 kg m
  • 3 Fluid viscosity: 2.56 N s m-^1 a) Assuming geometric similarity between the two systems, determine the volume of the large-scale bioprocessor with an impeller diameter of 600 mm. b) Calculate the impeller speed of the large-scale bioprocessor so that a constant power consumption per unit volume is maintained during scale-up between the small and large bioprocessor. Assume that both systems have turbulent flow. c) What are the principal assumptions of Richard’s sterilization method? d) With the aid of Table 1, for each bioprocessor calculate the holding time in minutes at 121°C needed to achieve a risk of contamination after treatment of one-in-ten thousand. Both bioprocessors have an initial microbial load of 10 7 microorganisms per ml. Assume that it takes 13 minutes to heat the contents of the bioprocessors from 100°C to 121°C and 17 minutes for the cooling cycle. Page 1 of 2…PTO 123 3.929 19. 124 4.881 2 4. 125 6.056 30. 126 7.506 38. 127 9.293 47.3 73 128 11.494 58. 129 14.200 73. ____________________________________________________^130 17.524^ 90. Table 1

T oC k min-^1 ∇

100 0.019 --- 101 0.025 0.044 - 102 0.032 0. 103 0.040 0. 104 0.051 0. 105 0.065 0. 106 0.083 0. 107 0.105 0. 108 0.133 0. 109 0.168 0. 110 0.212 0. 111 0.267 1. 112 0.336 1. 113 0.423 1. 114 0.531 2. 115 0.666 3. 116 0.835 3. 117 1.045 5. 118 1.307 6. 119 1.633 7. 120 2.037 10. 121 2.538 12. 122 3.160 15.