DNMT Inhibitor Screening: DNA Methyltransferase Assays at Creative BioMart, Slides of Biology

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DNA Methyltransferase

Inhibitor Screening

DNA methylation is one of the central epigenetic modifications in

the genome of higher eukaryotes, and involves the transferring of

a methyl group from S-adenosyl-L-methionine (SAM) to the C

position of cytosine, mostly within CpG dinucleotides. The

establishment and maintenance of DNA methylation patterns are

associated with DNA methyltransferases, mainly including

DNMT1, DNMT3a, and DNMT3b, where DNMT1 is the most

abundant DNMT in mammalian cells and acts as the primary

maintenance methyltransferase to keep the methylation of DNA

that is already established at the genome. DNMT3a and DNMT3b

are capable of adding de novo CpG methylation marks and thus

may dynamically regulate gene silencing, which is important for

embryonic development in epigenetic transcriptional regulation.

Two additional enzymes, DNMT2 and DNMT3L, may also have

more specialized but related functions. DNMT2 could methylate

tRNAAsp at C38 and DNMT3L as a catalytically inactive

regulatory factor of DNMTs is essential for the function of

An ever-increasing body of evidence has indicated many types of genetic disorders and a large number of human malignancies are closely related with abnormal DNA methylation due to the increase in the expression or activity of DNMTs. DNMTs inhibition may result in demethylation and expression of silenced genes. DNMT is a potentially predictive biomarker and therapeutic target in the diagnosis and treatment of various cancers, and DNMT inhibitors could therefore have potential applications in future oncology drug development. Creative BioMart is at the forefront of epigenetic drug discovery and aims at solving challenges by offering a panel of DNA methyltransferase screening assays for DNMT inhibitors screening and profiling. Creative BioMart offers DNMTs screening services, including DNMT1, DNMT3a and DNMT3b, by utilizing chemiluminescent or colorimetrical method. The core platform technologies and skilled work team at Creative BioMart make it available for our clients to develop an impressive early discovery pipeline focused on the DNMT class of targets. Trust us to promote you DNMTs-based lead optimization with the most competitive price and highest data quality in a

Procedure of DNA Methyltransferase Screening at Creative BioMart a. Chemiluminescent Method Highly specific antibodies that recognize methyl groups are employed in this protocol. SAM is first incubated with a sample containing assay buffer and purified recombinant DNMT. Acceptor beads, a primary antibody, and donor beads are subsequently added. The ultimate step is to read the Alpha-counts.

Features of DNA Methyltransferase Screening Services

at Creative BioMart

Simple, reliable, and consistent assay conditions

Rapid procedure that is completed within a few hours

Pioneering colorimetric assay without extraction and

chromatography

Flexible: manual or high throughput analysis due to the strip

microplate format

A broad range of services at Creative BioMart make it easy to

screen and profile your compounds against target DNMTs. Our

cutting-edge science team is dedicated to ensuring that our DNMTs

profiling services are implemented at the highest level of quality. We

are confident to provide high-efficiency service to meet your strict

project timelines on the basis of the commitment of prompt

Thank you

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https://www.creativebiomart.net/epigenetics/services/ epigenetic-drug-discovery/biochemical-assays/ methyltransferase-screening/dna-methyltransferase- screening/