dynamic light scattering dls analysis, Slides of Biology

Download dynamic light scattering dls analysis and more Slides Biology in PDF only on Docsity!

dynamic light scattering dls

analysis

Assessing the developability of drug candidates in the early stage of drug development is

important to reduce the risk of costly downstream failure. Dynamic light scattering (DLS), also

known as photon correlation spectroscopy, has become one of the main techniques for

evaluating the aggregation, stability, and viscosity of therapeutic proteins, monoclonal

antibodies, peptide drugs, and other biopharmaceuticals due to its ability to measure the size

distribution of macromolecules and nanoparticles. Moreover, this technique can also be

utilized to identify promiscuous inhibitors from hit compounds screened from small molecule

compound libraries.

Creative Biostructure has advanced DLS equipment that can provide fully automated DLS

services to complete some high-throughput screening programs. In the fully automated,

microplate reader format, DLS constitutes a high-throughput biophysical screening approach

for early assessment of the developability and formulation conditions of candidates. Compared

with the traditional cuvette-based DLS, high-throughput (HT)-DLS performed on microplates

can usually provide 10-100 times measurements.

Capabilities and Advantages of our MagHelix™ Dynamic Light Scattering (DLS) services:

We support high-throughput DLS based on microplates (96-, 384- or 1536-well microplates), which can quickly and

accurately perform an early evaluation of the developability and optimal formulation conditions of candidate

molecules.

We have many years of experience in using DLS to evaluate the size and size distribution of aggregates, including

small molecules, proteins, liposomes, virus-like particles, nanoparticles, etc.

The aggregation and stability of therapeutic proteins, therapeutic antibodies, nanosized colloidal gold, and other

biological agents can be detected with a very small amount of samples.

We can utilize DLS to identify promiscuous inhibitors from potential lead candidates. These promiscuous inhibitors

have concentration-dependent inhibitory properties while exhibiting non-drug-like characteristics.

The protein-protein, protein-nucleic acid, and protein-small molecule interactions can also be investigated by DLS.

As experts in X-ray crystallography, we use DLS to analyze the homogeneity of sample solutions before obtaining

high-resolution crystal structures.

Creative Biostructure has mastered a variety of popular biophysical approaches and techniques to provide a

wealth of information for the screening, validation, characterization, and optimization of potential candidates in

drug discovery programs. If you would like to know more, please feel free to contact us and our drug discovery

specialist can provide professional consultation for you.

Thank you!

Excerpt from:

https://drug-discovery.creative-biostructure.com/maghelix-

dynamic-light-scattering-dls-p