0
0.5
0 5 10 15
Absorbance
Time (min)
Time vs. Absorbance
Question
: Does temperature impact
the effectiveness of an enzyme?
•Experimental Set-Up: Preform an experiment using 1.5 mL of 2 mM
ONPG and 25 !L of β-galactosidase in a cuvette to measure the
absorbance of the solution. We will place the cuvettes into a water
bath at 37℃, and measure absorbance every two minutes, for 10
minutes, using the spectrometer. Do this 3 times.
•Hypothesis: As temperature
increases, enzyme activity
will also increase.
Data obtained from experiment.
Results of experiment in a Time vs. Absorbance graph
•Prediction: The absorbance of
the solution will be higher at a
lower time, and the reaction
will proceed at a much faster
rate.
•Null-Prediction: The
absorbance of the solution will
not change and will be virtually
the same as the control.
•Conclusion: The change in heat from 25 ℃to 37 ℃did not speed the reaction; the
absorbance was lower. Temperature does have an effect however it had the effect of
slowing the reaction down. We accept the null-hypothesis and reject our hypothesis.
•Null Hypothesis: As the
temperature increases, enzyme
activity will not change.
P value: .000019
