Oil Spill Bioremediation, Thesis of Financial Accounting

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Oil Spill Bioremediation SCI 207: Our Dependence Upon the Environment Oil Spill Bioremediation Introduction

Oil spill bioremediation is an especially important process that can help lessen the amount of pollution in our waters. Oil spills have been affecting the healthiness of waters and its creatures for many years. Even though it may seem like the major oil spills have played a big part in the polluted waters it is the small spills that cause a greater affect. We do not realize how much an oil spill can affect not just the water but everything living in it as well. The pollution can go all the way up the humans, it starts with the animals in the water. The flying animals then eat animals such as fish and become contaminated. If a human is to eat one of these birds, then now the human population is contaminated. We also must know where to dispose of oils the right way, the smallest amount of oil can run into the water drains and eventually end up in the ocean. Scientist coming up with bioremediation greatly helps with this issue but the best way to avoid all of this is to prevent oil spills in the first place. Take care of the environment and it will take care of you. Objectives paragraph: The objective of this lab is to simulate how the process of bioremediation works on oil spills. The Rid-X mixture will be used as the microorganisms and the tetrazolium will be used as the indicator to break down the oil. Showing this process works can help with the understanding of how harmful oil spills are and what we can do to help prevent the further pollution of bodies of water. Hypotheses: Tubes 1 and 2.

 Scissors  1 marker  10 3mL pipets  6 culture test tubes w/ caps  Phone for timer/camera Methods: Activity 1-

1. Before beginning this activity prepare the Rid-X solution and the tetrazolium solution.
2. This activity will take four days all together to complete.
3. Determine a hypothesis for each set of test tubes and begin activity.
4. For test tube one add the following ingredients in this order 2 mL of water, 10 drops of oil, 2 mL water, and 1 mL tetrazolium solution.
5. For test tube two add the following ingredients in this order 2 mL of water, 10 drops of oil, 2 mL microbial solution, and 1 mL tetrazolium solution.
6. For test tube three add the following ingredients in this order 2 mL water, 10 drops of oil, and 3 mL water.
7. For test tube four add the following ingredients in this order 2 mL water, 10 drops of oil, 2 mL microbial solution, and 1 mL water.
8. For test tube five add the following ingredients in this order 10 drops olive oil, 10 drops vegetable cooking oil, 2 mL microbial solution, and 1 mL tetrazolium solution.
9. For test tube six add the following ingredients in this order 10 drops olive oil, 10 drops vegetable cooking oil, 2 mL microbial solution, and 1 mL tetrazolium solution.
10. Mix each solution completely loosen the tops and let sit overnight.
11. When observing the tubes each day tighten the tops to avoid any spillage and loosen them back up when done.

12. Each day write down your observations and take photographs of tubes with each one clearly labeled. Results Observations/Data Tables Data Table 1. Tubes 1 and 2 Observations Day Tube 1 Tube 2 0 (Initial setup) The color is clear, and the oil is already beginning to settle at the top with some of the oil still throughout the solution. The color is a diluted milky color with the oil settling at the top 1 The color is still clear. There is some oil settled at the top, but it looks like there is still some spots of oil throughout the tube. The color is clear with a tannish tent to it. All the oil it settled at the top and the microbial substance is settled at the bottom with a pink color. 2 The color is clear with the oil spread throughout the tube. The color is pinkish color with oil settled at the top and the microbial at the bottom. 3 The color is clear, and it looks like most of the oil has settled at the top with a few spots throughout the tube. The color looks like a chalky pink color with the oil bubbled up at the top. The microbial solution has settled at the bottom with a pink tent. Data Table 2. Tubes 3 and 4 Observations Day Tube 3 Tube 4 0 (Initial setup) Color is clear with the oil settled at the top The color is a milky color with the oil settled at the top.

oils were trying to separate from each other. There were a lot of bubbles but there was a little separation. Just like all the other tubes the oil did settled all together at the top. What I have learned paragraph: While doing this lab I learned that there is a method that is being created to separate oils from the waters. I saw how this process can happen over a time. It is not something that can be done in an instant. Scientist are actively working on ways to preserve the microorganisms so that if a major spill does happen they are able to act fast before too much damage is done. Sources of error paragraph: When measuring out the substances it was difficult to get exact amounts with the plastic pipet. Because of that the amounts for each tube may have not been the same. I do not think that would have cause too much of a problem, but I am not sure of that either. If I had to do the lab again, I would find a better way to measure out everything. Future research paragraph: A question that I did have would be what would happen if the variable changed was to temperature or sunlight. I would like to have seen what would happened if sunlight made a difference in how fast the oil separated or how fast the solution reacted. Other than that, I did not see very much to change in this lab. To answer the question of change I would just do the lab over again changing the variables in tubes 5 and 6 again. References

Amr S. Ismail; Huda S. El-Sheshtawy; Nagla M. Khalil. In: Egyptian Journal of Petroleum, Vol 28, Issue 2, Pp 205-211 (2019); Elsevier, 2019. Language: English, Database: Directory of Open Access Journals. Bensel, Terry; Carbone Ian (2020). Sustaining Our Planet. Retrieved from https://content.ashford.edu/books/Bensel.5477.18.1/sections/title Atlas, Ronald; Bragg, James. Microbial Biotechnology. Mar2009, Vol. 2 Issue 2, p213-

221. 9p. 1 Color Photograph, 1 Black and White Photograph, 8 Graphs. DOI: 10.1111/j.1751- 7915.2008.00079.x. , Database: Academic Search Complete