LSU BIOL 4087 Exam 1 Questions With
Expert Answers
Q: EDTA
A: Inhibits metal-dependent proteases
Q: DTT
A: Keeps cysteine residues reduced; not used when dealing with antibodies
Q: Spectroscopy
A: Measures the quantity of proteins. Put sample in machine; if light passes through, there is not
a lot of protein; recorded as absorbance
Q: 240 nM
A: What number separates visible and UV light?
Q: 280 nM
A: What wavelength do we use to measure protein spectroscopy?
Q: 260 nM
A: What wavelength do we use for DNA/RNA spectroscopy?
Q: Protein purification
A: Sequence, shape, and size are very important because they determine solubility, charge,
polarity, and affinity
Q: Precipitating proteins
A: 1. Add salt to disrupt H bonds with protein and water
