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Microbe Mission 2017 Practice Test
Name: Date:
- Station 1
Match the labeled components from the microscope at this station with the function it performs.
Connects the body tube to the base.
- Brings the specimen into general focus.
- Flat platform where the specimen slide is placed.
- The lenses closest to the specimen.
- A rotating turret that houses the objective lenses.
- Adjusts the amount of light that reaches the sample. 7. Fine tunes the focus and increases the detail of the specimen. 8. Metal clips that hold the specimen slide in place. 9. Connects the eyepiece to the objective lenses. 10. The lens the viewer looks through to see the specimen. 11. Station 1
What type of microscope is at this station?
- What is the total magni cation power when using the objective used with oil immersion?
page 1
- What is the total magni cation when using this microscopes ocular and the largest objective?
- Station 2
Consider the following statements about various types of microscopes.
A. The image seen is two-dimensional. B. Lenses are made of glass. C. High magni cation with low resolution. D. Focusing is performed electrically. E. High magni cation and resolution. F. Specimen is mounted on aluminum stubs and coated in gold. G. Provides specimen contrast through use of electron scattering H. Focus is accomplished mechanically. I. Low magni cation. J. Specimen contrast is provided through light absorption or light re ection K. Specimen is mounted on a glass slide. L. The image seen is three-dimensional.
For the question, use the letters from the statements above to indicate which statements are true for the microscope type listed. List all of the statements that are true for the microscope type.
Compound Microscope
- Stereoscope
- Scanning Electron Microscope
- Transmission Electron Microscope
- Station 3
Use the information provided below to answer the question.
Under optimum conditions, E. coli cells double every twenty minutes. Since E. coli is a microbe, a growing colony is not visible without a microscope until several billion cells are present.
Assume that a plate has been streaked with E. coli and was incubated at optimal conditions for 8 hours. At the end of the 8-hour period the culture had 16,777,216 cells present in the sample.
A table prepared to demonstrate the growth in number of E. coli in a colony over time
Time (minutes)
Number of Cells
Time (minutes)
Number of cells 0 260 20 280 40 300 60 320 80 340 100 360 120 380 140 400 160 420 180 440 200 460 220 480 16,777, 240
How many individual E. coli cells were present in the initial sample?
- Station 3
Use the information provided below to answer the question.
Under optimum conditions, E. coli cells double every twenty minutes. Since E. coli is a microbe, a growing colony is not visible without a microscope until several billion cells are present.
Assume that a plate has been streaked with E. coli and was incubated at optimal conditions for 8 hours. At the end of the 8-hour period the culture had 16,777,216 cells present in the sample.
A table prepared to demonstrate the growth in number of E. coli in a colony over time
Time (minutes)
Number of Cells
Time (minutes)
Number of cells 0 260 20 280 40 300 60 320 80 340 100 360 120 380 140 400 160 420 180 440 200 460 220 480 16,777, 240
How many individual E. coli cells were present after 4 hours of incubation?
- Station 3
Use the information provided below to answer the question.
Under optimum conditions, E. coli cells double every twenty minutes. Since E. coli is a microbe, a growing colony is not visible without a microscope until several billion cells are present.
Assume that a plate has been streaked with E. coli and was incubated at optimal conditions for 8 hours. At the end of the 8-hour period the culture had 16,777,216 cells present in the sample.
A table prepared to demonstrate the growth in number of E. coli in a colony over time
Time (minutes)
Number of Cells
Time (minutes)
Number of cells 0 260 20 280 40 300 60 320 80 340 100 360 120 380 140 400 160 420 180 440 200 460 220 480 16,777, 240
How many individual E. coli cells would you expect to be present if the plate was left to incubate for another hour?
- Station 3
Use the following information to respond to question.
In 2014 a study was published in the European Journal of Dentistry regarding an evaluation of potential antibacterial e ects of pulp capping agents. In this study, researchers placed the bacterium Lactobacillus casei into direct contact with four di erent materials and then cultured the bacteria to determine whether or not the test materials had any a ect on its growth. Colony density was measured using an optic densitometer. Below is a graph summarizing their results.
Which material was the least e ective at retarding the growth of L. casei?
A. Kalzinol B. Calcimol LC C. DiaRoot D. Dycal
- Which material was the most e ective at retarding the growth of L. casei?
A. Kalzinol B. Calcimol LC C. DiaRoot D. Dycal
- At what time (hour) did the Kalzinol contacted sample enter the stationary phase?
- What phase of the bacterial growth curve was the Calcimol LC contacted sample in at hour 8?
- Does this graph depict arithmetic or geometric growth?
- Station 4
Match the following disease with the classi cation of microbe that causes it. Choose only one letter per answer. If multiple letters are used in a single response, it will be counted as incorrect; no partial credit will be given.
hepatitis Cholera Ergotism Malaria Kuru Mononucleosis Histoplasmosis Dental Caries Trichinosis Ringworm Paralytic Shell sh Poisoning Peptic Ulcer Disorder Ebola Hemorrhagic Fever Shingles
Algae Archaea (^) Bacteria (^) Fungi (^) Parasitic Worms Prions Protozoa (^) Viruses
- Station 6
A B C
Which image shows cocci bacteria?
A. A B. B C. C D. none
- Which image shows spirillium bacteria?
A. A B. B C. C D. none
- Using the reference within the image, approximately how long (in micrometers) are the bacilli in the sample above?
- Which image demonstrates the morphology of Mycobacterium tuberculosis?
A. A B. B C. C D. none
- Station 6
Complete the following statement.
When utilizing the Gram stain technique, Gram positive bacteria will stain.
- When utilizing the Gram stain technique, Gram negative bacteria will stain.
- In Gram staining two stains are used: crystal violet and.
- MRSA is an example of a Gram (select one: positive or negative) bacteria.
- Gram (select one: positive or negative) bacteria are more receptive to antibiotic treatment.
- Gram (select one: positive or negative) bacteria have a thick peptidoglycan layer.
- This chemical is used during the Gram staining technique to help the crystal violet stain bind and become trapped within a cell.
- Station 7
Respond to the following statement with either a T (if the statement is true) or F (if the statement is false).
ID 50 is the approximate does of a chemical or microbial population that produces illness in at least 50% of animals.
- The arthropod vector of Lyme Disease is the ea.
- Genetic variation among bacterial populations may result from transduction and/or meiosis.
- Fungi are used in the production of baked goods.
- Plant-like Protists are responsible for production of a signi cant amount of the oxygen in Earth's atmosphere.
- Station 8
Identify the microbe using the following dichotomous key.
- Station 8
A B C D
Which microbe pictured above is associated with buttermilk production?
A B C
a) What is the approximate length of organism B?
b) Which organism, A or B is larger?
c) What is the approximate length of the nucleus, the darkly staining material, in gure B?
d) To what speci c group of protozoans does organism C belong?
e) What is the approximate length of organism C?
f) What is the approximate width of organism C?
- Station 10
Match the following word with the correct de nition
Dieocious Monoeicious Intermediate Host Scolex De nitive Host Parasite Phylum Nematoda Phylum Platyhelminths Proglottids
(^) The host in which larval development occurs. (^) Hooks/suckers that help tapeworms hold onto the hosts tissue. A species that on one organism can exist male and female parts. An organism that lives on or in a host organism and harms that organism. (^) Segments on a tapeworm that have both male and female reproductive organs. (^) Examples are ukes and tapeworms (^) A species that has both male and female individuals. The host in which adulthood and mating occur. Examples are pin worms, Ascaris, and hookworms
- Station 11
Break Station
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Microbe Mission 2017 Practice Test 12/2/
Answer: C
Answer: A
Answer: K
Answer: J
Answer: L
Answer: I
Answer: B
Answer: D
Answer: H
Answer: G
Answer: Compound
Answer: 100X
Answer: 400X
Answer: 1, 2, 3, 8, 11
Answer: 2, 8, 9, 10, 12
Answer: 4, 5, 6, 7, 12
Answer: 1, 4, 5, 7
Answer: A
Answer: B
Answer: D
Answer: 30 m
Answer: Chloroplast
Answer: 1
Answer: 2051
Answer: 134,217,
Answer: D
Answer: C
Answer: D
Answer: 13
Answer: log
Answer: Geometric
Answer: 1H, 2C, 3D, 4G, 5F, 6H, 7D, 8C, 9E, 10D, 11A, 12C, 13H, 14H
Answer: 1F, 2F, 3E, 4C, 5A, 6B, 7D
Answer: 1A, 2A, 3C, 4P, 5A, 6P, 7A, 8P, 9A, 10P, 11P
Answer: B
Answer: C
Answer: 5 m
Answer: A
Answer: Violet
