Study with the several resources on Docsity
Earn points by helping other students or get them with a premium plan
Prepare for your exams
Study with the several resources on Docsity
Earn points to download
Earn points by helping other students or get them with a premium plan
The fine adjustment knob can be used when the image is almost in focus to fine tune. When changing the lens make sure to alter the condenser aperture iris ...
Typology: Schemes and Mind Maps
1 / 3
This page cannot be seen from the preview
Don't miss anything!
Department of Chemistry Teaching Laboratories
View selector Light intensity lever Fine adjustment knob Slide holder Filter mount (Polarising filter fitted) Eyepieces Camera Field iris diaphragm ring Coarse adjustment knob Condenser aperture iris diaphragm ring (^) Stage adjuster Y-axis knob X-axis knob Objective lens
Ensure that the microscope and its connected PC are turned on, open the software as described overleaf. Set the view selector to show the image through the eyepieces and the camera, or just the camera. This should be either half way or all the way out. Prepare slide as instructed, place on the stage ensuring that the slide is held in place by the slide holder. Set the light intensity lever to ~ 3 to begin with. You can press the preset button which is set to light level most suitable for photomicrography. Fully open the field iris diaphragm ring to allow the maximum amount of light to reach the sample. This can be adjusted later. Set the condenser aperture iris diaphragm ring to 0.10 on the scale, this is set for the lowest magnification objective lens which you will start with.
There are three different objective lenses available on the microscope these are shown below. These details are indicated on the lenses themselves. Lens type Magnification Condenser aperture Tube length Coverslip thickness Planar 4x 0.10 ∞ - Achromatic 10x 0.25 ∞ - Achromatic 40x 0.65 ∞ 0. Starting with the lowest magnification lens, raise the stage to the highest level using the coarse adjustment knob. Ensure the sample is in the field of view using the Y- axis and X-axis knobs to adjust the position of the slide. From there lower the stage away from the lens until the image is in focus. If more magnification is needed, then as long as the image is in focus you should be able to swap the lens out for the next magnification level. Make sure that the lens isn’t going to come into contact with the slide each time you swap and always focus away from the lens. The fine adjustment knob can be used when the image is almost in focus to fine tune. When changing the lens make sure to alter the condenser aperture iris diaphragm ring to match the new lenses rating.
