Microscope Use and Expectations, Exercises of Biology

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3. Plug in the microscope and/or turn it on.
4. Revolve the nosepiece until the low-power objective lens clicks into place.
5. Place a slide on the stage. Center the specimen over the opening on the stage. Use the stage clips to hold the slide in place.
6. Look at the stage from the side. Carefully turn the coarse adjustment knob to raise the stage until the low-power objective almost touches the slide.
7. Looking through the eyepiece, very SLOWLY turn the coarse objective knob until the specimen comes into focus. CAUTION: If you turn too quickly the slide could hit and break the objective lens. MAKE SURE THE LENS DOES NOT HIT THE SLIDE.
8. Use the fine focus knob to bring the specimen into perfect focus.
9. To switch objective lenses, look at the microscope from the side and carefully revolve the nosepiece until the appropriate objective is in place.
10. Use the coarse and fine adjustment knobs to adjust the image at each of the magnifications. CAUTION: NEVER , EVER, use the coarse adjustment knob with the high power objective. This could break the lens.

Determining Total Magnification

Multiply the power of the ocular lens, by the power of the objective lens. OCULAR LENS = 10X OBJECTIVE LENS = 4X TOTAL MAGNIFICATION = 40X