Mempro™ Nanodisc Preparation Services: Design, Synthesis, Immobilization and More, Slides of Biology

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Nanodisc Immobilization

Creative Biostructure has focused on Nanodisc technology for years ， our team has

established an advanced Mempro™ Nanodisc preparation platform to provide various

services including design, synthesis and immobilization, etc. Scientists from Creative

Biostructure are confident in providing high-quality Mempro™ Nanodisc preparation

services to meet all your demands.

Nanodiscs are soluble nanoscale phospholipid bilayers, which are composed of

membrane scaffold proteins (MSPs). Nanodiscs can maintain membrane proteins in

solution, and offer an inartificial nature phospholipid bilayer environment in which the

incorporated target keeps stable. Nanodiscs are being used to study isolation,

purification and solubilization of membrane proteins and also is an advanced tool to

learn interacting companions of membrane embedded molecules.

Mempro™ Nanodisc Design Services

The MSP used for generating Nanodiscs is based on the sequence of human

serum apolipoprotein AI. A bilayer domain is formed by the combined

phospholipid, meanwhile, the edges of the discoidal structure are warped around

by two molecules of MSP in a belt-like configuration. To get highly homogeneous

Nanodiscs, a specific ratio of phospholipid to MSP is necessary during the

assembly process based on the MSP belt-length/Nanodisc diameter. The

detergent used for initial solubilization is important as well, of which sodium

cholate is commonly used. This detergent is perfect for the lipid fraction and

generally present at a 2:1 mole ratio to total PL, or higher. Creative

Biostructure can design Nanodisc in various sizes with our Mempro™ Nanodisc

design services.

Mempro™ Nanodisc Synthesis Services

Nanodiscs are assembled by a compound of detergent/phospholipid micelles and

MSPs. In all cases, the assembly process is begun by removal of detergent, and

the removal process is usually achieved by dialysis or treatment with porous

polystyrene beads. Besides, it’s both detergent and temperature-dependent

when removing the detergent by beads. Creative Biostructure provides various

Nanodisc synthesis services with the accumulated experience, we can perform

Nanodisc with highly suitable temperature, ratio of phospholipid to MSP and ideal

Mempro™ Nanodisc Immobilization Services

The ability of modifying the MSP sequence and sticking on different tags at

particularly engineered sites offers probability for the immobilization of

membrane bilayers and membrane-associated molecules for affinity

chromatography media and measurements on protein chips. Through this

immobilization, membrane and its receptors are part of the vesicles bound by

hydrophobic or specific anchoring through binding of molecules built-in the

phospholipid bilayer.

Mempro™ Peptide Nanodisc Services

In peptide nanodiscs, the lipid bilayer is covered by amphipathic peptides take

the place of two MSPs. Peptide nanodiscs have a similar structure as MSP

nanodiscs and usually their peptides align in a double belt. Peptide nanodiscs

present a higher polydispersity when stabilise membrane proteins, however have

a less structural stability than MSP nanodiscs.

Creative Biostructure provides other various Mempro™ Nanodisc technology

services. Please feel free to contact us for a detailed quote.

Thank you!

Excerpt from:

https://www.creative-biostructure.com/mempro

%E2%84%A2-nanodisc-preparation-services-419.htm