Nanodisc preparation
Creative Biostructure has focused on Nanodisc technology for years，
our team
has established an advanced Mempro™ Nanodisc preparation platform to
provide various services including design, synthesis and immobilization, etc.
Scientists from Creative Biostructure are confident in providing high-quality
Mempro™ Nanodisc preparation services to meet all your demands.
Nanodiscs are soluble nanoscale phospholipid bilayers, which are composed
of membrane scaffold proteins (MSPs). Nanodiscs can maintain membrane
proteins in solution, and offer an inartificial nature phospholipid bilayer
environment in which the incorporated target keeps stable. Nanodiscs are
being used to study isolation, purification and solubilization of membrane
proteins and also is an advanced tool to learn interacting companions of
membrane embedded molecules.
Mempro™ Nanodisc Preparation Services
Figure 1. Illustrations of Nanodisc structures. (FEBS Letters 2010)
Mempro™ Nanodisc Design Services
The MSP used for generating Nanodiscs is based on the sequence of human
serum apolipoprotein AI. A bilayer domain is formed by the combined
phospholipid, meanwhile, the edges of the discoidal structure are warped
around by two molecules of MSP in a belt-like configuration. To get highly
homogeneous Nanodiscs, a specific ratio of phospholipid to MSP is necessary
during the assembly process based on the MSP belt-length/Nanodisc diameter.
The detergent used for initial solubilization is important as well, of which
sodium cholate is commonly used. This detergent is perfect for the lipid
fraction and generally present at a 2:1 mole ratio to total PL, or higher. Creative
Biostructure can design Nanodisc in various sizes with our Mempro™
Nanodisc design services.
Mempro™ Nanodisc Synthesis Services
Nanodiscs are assembled by a compound of detergent/phospholipid micelles
and MSPs. In all cases, the assembly process is begun by removal of
detergent, and the removal process is usually achieved by dialysis or
treatment with porous polystyrene beads. Besides, it’s both detergent and
temperature-dependent when removing the detergent by beads. Creative
Biostructure provides various Nanodisc synthesis services with the
accumulated experience, we can perform Nanodisc with highly suitable
temperature, ratio of phospholipid to MSP and ideal detergent.
Mempro™ Nanodisc Immobilization Services
The ability of modifying the MSP sequence and sticking on different tags at
