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NOCTI test
National Institute of Health - correct answer NIH Center for Disease Control - correct answer CDC Occupational Safety and Health Administration - correct answer OSHA Environmental Protection Agency - correct answer EPA National Institute of Health - correct answer In charge of laboratories that use or contain recombinant DNA. Center for Disease Control - correct answer In charge of laboratories that use or contain infectious agents Occupational Safety and Health Administration - correct answer monitors work conditions and eliminating physical and health hazards at the work place. Environmental Protection Agency - correct answer In charge of wastes that labs generate and store such as hazardous waste. hazardous substance - correct answer Material/substance that poses physical or health hazard. Biohazard - correct answer Any organism capable of replication and is capable of causing disease in human, animal, or plant. Carcinogen Toxic Corrosive
Etc. - correct answer Characteristics of health hazards Explosive Flammable Unstable(reactive) Etc. - correct answer Characteristics of physical hazards Spill kit - correct answer What should be available when using chemical, biological, or radioactive agents? Infectious agent - correct answer organism capable of producing infection or disease in human, animal or plant Recombinant DNA - correct answer Molecules constructed outside living cells and can replicate in a living cell. (We mutate DNA) Additional lab specific training - correct answer When using infectious agents what is required by you? True - correct answer True or false- infectious agents can cause illness or death to BOTH people and animals? Biosafety levels - correct answer Set of precautions required to isolate dangerous biological agents in a laboratory. Containment - correct answer Confinement of a biohazardous agent in order to prevent or limit its contact with people and/or the environment. Chemical Hygiene Plan - correct answer CHP When a chemical is in a laboratory there must be a CHP to communicate the hazards, procedures, equipment, PPE etc. - correct answer What is a chemical hygiene plan?
Other potentially infectious material - correct answer OPIM exposure control plan - correct answer plan that outlines specific work practices to prevent exposure to infectious material and identifies step-by-step procedures to follow when exposures do occur Personal Protective Equipment - correct answer PPE Equipment necessary to protect yourself from hazardous and biohazardous materials - correct answer What is PPE? Safety glasses, gloves, respirator - correct answer What type of PPE should you use when working with chemicals? safety glasses, gloves, lab coat, respirator - correct answer What type of PPE should be used when dealing with biological agents and/or rDNA? Zoonotic - correct answer disease that can be transmitted between animals and humans Gloves, lab coat, safety glasses, respirator, shoe covers - correct answer What type of PPE should be used when dealing with animals? Arthropods - correct answer Insects Gloves, safety glasses, lab coat - correct answer What type of PPE should you use when dealing with Arthropods? Do NOT recap needle - correct answer What's the one thing to remember when using a needle (sharp)?
A rigid, puncture and leak-resistant container that will keep moisture out - correct answer Where do sharps go after being used? The word biohazard or infectious waste - correct answer What are sharps containers labeled with? Must be recorded in the sharps log - correct answer What happens if injury occurs with a sharp? 4 - correct answer How many risk groups (RG) are there? RG1 - correct answer •Not associated with disease in healthy adult humans •No or low individual and community risk RG2 - correct answer •Associated with disease •Rarely serious •Preventative measures or treatment often available •Moderate individual risk •Limited community risk •Most common for clinical research RG3 - correct answer •Associated with serious or lethal disease •Preventative measures or treatment may be available •High individual risk •Low community risk RG4 - correct answer •Likely to cause serious or lethal disease •Preventative measures or treatment are not usually available •High individual risk •High community risk
high efficiency particulate air filter(in BSC) - correct answer What is a HEPA filter? True - correct answer True or false- you use PPE when working with a BSC? Do not bring contaminated materials out of the cabinet - correct answer What is important to remember when using infectious agents in a BSC? Machine used to treat infectious material and rDNA. - correct answer What is an Autoclave? By reaching high temps - correct answer How do autoclaves sterilize the agent? Autoclave red bag and spore strip - correct answer What do you put the infectious material in when using an autoclave? To verify that the heat treatment was successful (also verify that a color change took place) - correct answer What is the point of using a spore strip when autoclaving materials? They are incinerated - correct answer What happens to used sharps to get rid of them? You have to log it and it must be maintained for 3 years - correct answer What do you have to do after autoclaving something? Laboratory - correct answer Secondary barrier example Decontamination - correct answer The removal or cleansing of dangerous chemicals and other dangerous or infectious materials. (Includes sterilization and disinfection) standard operating procedure (directions) - correct answer SOP
Evacuate area Seek medical attention if necessary MUST RECORD IN SPILL LOG - correct answer What do you do if a spill occurs? Toxic Corrosive Ignitable Flammable Oxidizer - correct answer Hazardous Waste characteristics biohazardous waste - correct answer Waste that is considered infectious because of its biological nature True - correct answer True or false- biohazardous waste can be disposed in regular trash once it is non infectious? Biotechnology - correct answer A form of technology that uses living organisms, usually genes, for human benefit to make a product or solve a problem Fermentation Selective breeding Use of antibiotics - correct answer Historical examples of biotechnology gene cloning Genetic engineering Recombinant DNA - correct answer Modern examples of biotechnology Microbial Biotechnology - correct answer manipulation of microorganisms such as yeast and bacteria
Bioprospecting - correct answer Rich and valuable sources of new genes,proteins and metabolic processes with important applications for human benefits Creates more food for us - correct answer How does modified salmon help humans? Medical Biotechnology - correct answer Involved with the whole spectrum of human medicine Preventive medicine Diagnosis of health and illness Treatment of human diseases New information from Human Genome Project Gene therapy Stem cell technologies Regulatory Biotechnology - correct answer All activities involved in regulating the final quality of a product Quality Assurance (QA) - correct answer All activities involved in regulating the final quality of a product Quality Control (QC) - correct answer Involved in development systems to ensure that products or services are designed and produced to meet or exceed customer requirements and expectations. Human Genome Project - correct answer Research on the function of human genes and controlling factors that regulate genes human proteome - correct answer Collection of proteins responsible for activity in a human cell single nucleotide polymorphism - correct answer SNP stands for
Single nucleotide changes in DNA sequences that very from individual to individual.
- correct answer What are single nucleotide polymorphisms? Will help identify genes involved in medical conditions like arthritis, cancer, heart disease - correct answer What is the benefit of SNP's? Microarray - correct answer Compare patterns of human DNA binding between patients DNA and DNA on microarray to reveal patients SNP patterns Pharmacogenomics - correct answer Customized medicine tailor designed to the genetic profile of a patient Metabolomics - correct answer A snapshot of small molecules produced during cellular metabolism Nanotechnology - correct answer Small particles that can deliver drugs to cells Gene therapy technology - correct answer Replacing or augmenting defective genes with normal copies of the gene Example- what are the long term effects of introducing extra genes in humans? - correct answer What are some Obstacles of gene therapy technology? small interfering RNA (siRNA) - correct answer emerging technology to silence genes that are involved in disease progression
