Polyomaviridae - Human Resource - Lecture Slides, Slides of Human Resource Management

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DNA Synthesis by Polyomaviridae is

Bidirectional

Remove RNA, fill gaps, seal

Polyomaviridae DNA Synthesis

Leading strand presynthesis complex Lagging strand Rf-C binds 3’ R-D pcna is next Remove RNA, fill gaps, seal 42

The Replication Machine

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Problems in Replication

Catenated molecules 45

DNA Priming

Priming via a specialized structure

Parvoviruses self prime, form a template primer

Their genomic DNAs are ss of both + and - polarity and they contain Inverted Terminal Repeats start here - but how to get to the end? 47

A dependovirus!

No pol α, uses Inverted Terminal Repeat to self-prime

Requires pol δ, Rf-C and PCNA

Rep78/68 proteins are required for initiation and resolution

• endonuclease, helicase, binds 5’ terminus

No replication fork!

AAV Replication is Continuous

ITR

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Protein Priming

Adenoviridae - -Precursor to terminal protein (pTP) -Ad DNA pol links α-phosphoryl of dCMP to OH of a S residue in pTP -Added only when protein primer is assembled with DNA pol into preinitiation complex at ori -3’OH primes synthesis of daughter strand 50