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In these Lecture Slides of HRM, the Lecturer has discussed the following fundamental concepts of Human resource Management : Replication, Modes, Leading, Replication Fork, Strand Displacement, Adenoviruses, Parvoviruses, Poxviruses, Papillomaviruses, Leading Strand Replication
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21 Replication Fork Strand displacement (primer) Papillomaviruses Polyomaviruses Herpesviruses Retroviral Proviriuses Adenoviruses (protein) Parvoviruses (DNA hairpin) Poxviruses (hairpin Primer 5โ 3โ 3โ 5โ 5โ 3โ Leading Lagging
Initiation from a single ori, requires expression of Tag
Replicate as covalently closed circles
Leading strand replication occurs via extension from an RNA primer
Lagging strand replication is delayed until the replication fork has moved
How to fill in the gaps? 22
a multifunctional virus-specified early protein 24
Between pE and pL AT rich LT binding sites, SP1 sites Nucleosome free - Why? Late promoter 25
Leading strand DNA synthesis is continuous
Lagging strand DNA synthesis is discontinuous
Direction of synthesis off of either template strand is the same 27
28 Two LT hexamers bind LT Binding A/T site II EP 1 ATP + LT
30 Binding of Rpa occurs ATP ADP + Pi 3 RPA
