MODULE 8: RECOMBINANT PROTEIN
EXPRESSION SYSTEMS
1. Distinguish between pBR322 and pUC19 vectors
2. What are shuttle vectors? Give an example.
3. Understanding the mechanism of regulation of lactose metabolism in E.
coli paved way for the development of inducible prokaryotic expression
vectors – Explain
4. How are bacteriophages engineered to become expression vectors? Give
an example.
5. What are the selection markers normally used in:
a) E. coli expression vectors b) yeast expression vectors
6. Mention the merits and demerits of prokaryotic and eukaryotic expression
systems
7. Name two popular yeast expression systems that are widely used for
recombinant protein production
8. What is baculovirus? How is it useful in recombinant protein production?
9. What are the key features of an eukaryotic expression vector? Give two
examples of mammalian expression vectors.
10. Distinguish between transient and stable expression of genes in
mammalian cells.
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