Protein Purification Lecture Notes, Slides of Biochemistry

A lecture on protein purification, which is a process of isolating and purifying proteins from biological materials. The lecture covers the organization of proteins, primary, secondary, tertiary, and quaternary structures, and the steps of protein purification and analysis. It also discusses the properties of proteins that can be used to isolate them, such as solubility, molecular size, ionic charge, and binding specificity. The lecture notes include diagrams and examples of different protein purification procedures.

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2022/2023

Uploaded on 03/14/2023

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BIOCHEMISTRY 1
2ND CLASS
UNIVERSITY OF ANBAR-COLLOGE OF SCIENCE
BIOLOGY DEPARTMENT
2020-2021
Protein purification
Lecture five(5)
Hameed Hussein Ali
Chemistry Department
College of Science 1
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BIOCHEMISTRY 1

ND

CLASS

UNIVERSITY OF ANBAR-COLLOGE OF SCIENCE

BIOLOGY DEPARTMENT

Protein purification

Lecture five( 5 )

Hameed Hussein Ali

Chemistry Department

College of Science

Purify Characteristic: Procedure: Charge 1. Ion exchange chromatography

  1. Electrophoresis
  2. Isoelectric focusing Size: 1. Dialysis and ultracentrifugation
  3. Gel electrophoresis
  4. Gel filtration (size exclusion) chromatography Specificity: 1. Affinity chromatography Polarity: 1. Adsorption chromatography
  5. Paper chromatography
  6. Reverse-phase chromatography
  7. Hydrophobic chromatography