1). (10 Points). Transposable elements are useful tools for performing genetic
experiments.
(5 Points). List 5 different uses of transposon insertions.
Transposons can be found at a large number of sites in a chromosome (providing linked,
selectable markers).
Interrupted genes (almost) always are inactive.
The phenotype of the insertion is completely linked to drug resistance in genetic crosses.
Insertion mutations can revert by precise excision and loss of drug resistance.
Insertions are usually polar.
Transposons provide a portable region of homology.
Mud phages and other elements can be used to make operon and gene fusions.
Others possible.
(5 Points). Describe 2 different ways that have been used to introduce transposons into
cells.
Phages that contain the transposon but cannot replicate in the host
Suicide plasmids (introduced by conjugation or electroporation)
EZ Tn (introduced by electroporation)
Others possible