Bacterial Replication
I. DNA Structure
II. Mechanism of DNA Replication (Building a Replication Fork)
Primers Templates Accessories
Polymerases, Editors, Clamps, Clamp Loaders, Helicases
Messelson-Stahl Experiment
Leading and Lagging Strand, Okazaki Fragments
Proofing-reading what was written: Mismatch Repair, DnaQ
III. Replication Initiation, Termination, and the Cell Cycle
Origin of Replication
Termination of Replication
IV. Supercoiling and Packaging
Compartmentalization
Topoisomerases, Accessibility, Organization
V. Antibiotics that Block Replication
VI. Molecular Biology and DNA manipulation
Restriction Endonucleases
Cloning, DNA Libraries
Hybridizations, Southern Blots
PCR, polymerase chain reaction
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