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Transformation - Microbial Genetics - Lecture Notes, Study notes of Genetics

Biju Patnaik University of TechnologyGenetics

Biochemistry of Replication, Replication Fidelity, Translation, Mutants, Mutation Rates, Mutational Selection and Screens, Conjugational Analysis, Viral Transduction, Phage Genetics, Molecular Mechanisms of Recombination and Molecular Mechanisms of Repair are major topics of Microbiology Genetics. This lecture includes: Transformation, Griffith Experiment, Natural Vs. Induced Transformation, Pheromones, Recombination With Homologous Sequences, Electroporation, Heat Shock Induction, Gene Knockout

Typology: Study notes

2012/2013

Uploaded on 08/21/2013

shahi
shahi 🇮🇳

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Transformation
I. Terms and Discovery
Natural vs. Induced Transformation
Competence, Donor, Recipient, Transformant
Discovery- the Griffith Experiment
II. Competence (in B. subtilis)
Regulation
comP, comA
Pheromones
comX, comQ
Timing
Linear DNA Only (so plasmids don't get into naturally competent cells)
III. DNA Uptake
Species specific vs non specific uptake (depends upon the bacteria)
Mechanism of uptake
Double stranded DNA binds
Degraded into single stranded DNA when/soon after it enters the cell
Recombination with homologous sequences
transformasomes
IV. Why take up foreign DNA?
Nutrition?
Repair?
Advantages to Sexual Reproduction?
V. Induced Competence
Calcium/Heat Shock Induction
Electroporation
Induced Competence allows uptake of Double Stranded DNA
VI. Mapping/Gene Knockouts/Recombination
Using competence as a tool
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Transformation

I. Terms and Discovery Natural vs. Induced Transformation Competence, Donor, Recipient, Transformant Discovery- the Griffith Experiment II. Competence (in B. subtilis ) Regulation comP , comA Pheromones comX , comQ Timing Linear DNA Only (so plasmids don't get into naturally competent cells) III. DNA Uptake Species specific vs non specific uptake (depends upon the bacteria) Mechanism of uptake Double stranded DNA binds Degraded into single stranded DNA when/soon after it enters the cell Recombination with homologous sequences transformasomes IV. Why take up foreign DNA? Nutrition? Repair? Advantages to Sexual Reproduction? V. Induced Competence Calcium/Heat Shock Induction Electroporation Induced Competence allows uptake of Double Stranded DNA VI. Mapping/Gene Knockouts/Recombination Using competence as a tool

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