C๐ฎ๐ป ๐๐ผ๐ ๐ฒ๐
๐ฝ๐น๐ฎ๐ถ๐ป ๐๐ต๐ฒ ๐ฝ๐ฎ๐๐ต๐ผ๐ด๐ฒ๐ป ๐๐ฒ๐๐ ๐ถ๐ป ๐ ๐๐ง?
Detecting and quantifying specific microorganisms that may be present in pharmaceutical
products, including bacteria, fungi, and pathogens.
๐ฆ๐ฎ๐บ๐ฝ๐น๐ฒ ๐: Take 10 g of sample and add to SCDM 90 ml 1:10 (TAMC and TYMC).
๐ฆ๐ฎ๐บ๐ฝ๐น๐ฒ ๐: Take 10 ml from sample A and add it to 90 ml SCDM at a 1:100 ratio (pathogen test).
๐ฆ๐ฎ๐บ๐ฝ๐น๐ฒ ๐: Add 90 ml of SCDM to a 10g/ml sample (for the salmonella test).
Sample A discard
Samples B and C incubate at 30-35ยฐC for 24 hours.
๐ฃ๐ฎ๐๐ต๐ผ๐ด๐ฒ๐ป ๐๐ฒ๐๐.
๐. ๐ฐ๐ผ๐น๐ถ ๐๐ฒ๐๐.
Add 1ml of sample B to the MCB and incubate at 42-44 ยฐC for 48 hours. Then, streak on an MCA
plate and incubate for 72 hours.
Color indication: brick red
๐ฆ๐ฎ๐น๐บ๐ผ๐ป๐ฒ๐น๐น๐ฎ ๐ง๐ฒ๐๐
Add 0.1ml of sample C to the 10mL RVSEB and incubate at 30-35ยฐC for 48hrs. Then, streak on
an XLDA plate and incubate for 48 hours at 30-35ยฐC.
Indication with or without black center.
๐ฃ๐๐ฒ๐๐ฑ๐ผ๐บ๐ผ๐ป๐ฎ๐ ๐ง๐ฒ๐๐
Incubate the sample B streak on a CA plate at 30-35 degrees Celsius for 72 hours.
Indication: fluorescent green color.
๐ฆ๐๐ฎ๐ฝ๐ต๐๐น๐ผ๐ฐ๐ผ๐ฐ๐ฐ๐๐ ๐ฎ๐๐ฟ๐ฒ๐๐ ๐ง๐ฒ๐๐
Incubate the sample B streak on an MSA plate at 30-35 degrees Celsius for 72 hours. Indication
golden yellow color colonies.
All the test according to specification