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size exclusion

chromatography

Creative Biostructure provides custom Size-exclusion Chromatography (SEC) Service for biology

macromolecule purification.

Size-Exclusion Chromatography (SEC), also called Gel Filtration Chromatography, is a widely

applied separation technique based on the size of bio-macromolecules. SEC can be used to

separate many kinds of bio-molecules including but not limited to recombinant proteins,

enzymes and antibodies, nucleic acids, polysaccharides, etc. Through SEC, the components of

samples are separated by their different molecular size as they pass the SEC medium filled in a

column. Unlike other kinds of chromatography (for example: ion-exchange chromatography

(IEX) or affinity chromatography (AC), SEC doesn't need the binding reaction between sample

components and chromatographic medium to proceed the separation, so the sample buffer

components don't affect the separation directly. What's more, buffer, temperature, pH value

and other conditions can be varied to suit different kinds of samples or for further purification

requirements. SEC can be performed directly before or behind other chromatography.

SEC is an ideal method for samples that are sensitive to changes of buffer conditions such

as metal ion concentration, pH value, etc. To perform SEC, a packed bed is established by

packing the size exclusion medium into an SEC column. The packed SEC column is

equilibrated with SEC buffer to maintain the space between the medium particles and the

pores of the matrix were totally filled with SEC buffer. Samples are injected into the column

and then they flowing pass the packed bed, during which the separation occurs. Components

of samples are eluted isocratically without using different buffers. SEC can be performed in

the cold room or 37°C to meet the requirements of experiments. Biology macromolecules can

be collected in any SEC buffer with the presents of cofactors, detergents, essential metal

ions, denaturants, etc.

With years of experience on macromolecule purification, Creative Biostructure provides

custom size-exclusion chromatography service with high quality for various kind of bio-

macromolecule purification. Various purification systems such as AKTA and Bio-Rad are

available to choose. Our science team will establish optimal SEC strategies for individual

cases to provide you the best experiment result.

We can also provide other chromatography and purification services including but not limited

to:

Affinity Chromatography

Ion-exchange Chromatography

Hydrophobic Interaction Chromatography

Reversed Phase Chromatography

TFF/Diafiltration

Please feel free to contact us to discuss your project!

Thank you!

Excerpt from:

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