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Notes of video 12 on kinases in biology.
Typology: Summaries
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Video 12 Notes - Kinases
โ DNA is a linear template of four bases: [ Adenine, Guanine, Cytosine, and Thymine. ]
โ The speed with which we can sequence DNA is incomprehensible. โ Information lies within the linear template of DNA.
โ RNA is also a linear template of four bases: [ Adenine, Guanine, Cytosine, and Uracil. ]
โ Information lies within the linear template in RNA as well. โ Proteins are made up of 20 different amino acids, and the chemical properties of those amino acids are fairly different. โ Proteins get modified when they get made. โ With DNA and RNA, transcription transcribes the DNA into RNA. โ A protein sequence defines the chemical composition of that structure. โ To understand how a protein works, you need to have a structure, so you know where the amino acids are and how they work together to create an active and functional protein. โ Understanding proteins are much more complicated than reading DNA and RNA templates. โ The building blocks or the atoms that make up the protein: Carbons, Nitrogens, Oxygens, Hydrogens, Sulphur, and Phosphates. โ Frank Westheimer: one of the chemists that studied phosphoryl-transfer. โ Westheimer elucidated the importance of phosphates for biology.
Frank Westheimer made two points:
(The bonds linking the phosphate groups have high potential energy!
โ The average 70-kilogram person turns over 40 kilograms of ATP a day. โ What Westheimer did not address at all was protein phosphorylation as a mechanism for regulating biology. โ Krebs and Fischer were the first to demonstrate in the late 1950s, that phosphorylation was important for the regulation of proteins. โ Glycogen particles: when we have a carbohydrate-rich meal, the liver takes up the glucose and you make glycogen, and store it there. When you wake up in the morning, you have mobilized some of that glycogen into your bloodstream, so that your brain and the rest of your body still get glucose. โ In our bodies, an enzyme called glycogen phosphorylase makes and breaks down glycogen as a fundamental part of metabolism. โ When the glycogen phosphorylase is phosphorylated, it becomes active. Whereas when it is not phosphorylated, it becomes inactive. โ A protein kinase uses ATP and transfers the gamma phosphate into a protein. โ Phosphates are going on and off of your proteins, all the time and they are commonly referred to as molecular switches for all of biology. โ The whole process of mitosis is primarily mediated by kinases and phosphatases that get turned on and off, and they allow the start and stop of different stages of mitosis.
Src Discovery:
โ 1970: Src was first discovered as an oncogene in chickens from Rous Sarcoma Virus, which causes cancer in chickens. โ 1978: It was discovered that Src also had protein kinase activity. โ 1979: Scr was cloned, so now you have the sequence of Scr. โ In 1980, it was discovered that Src was also a protein kinase.
โ Tyrosine is another amino acid that can be phosphorylated and is important for biology and disease. โ Kinases in general add phosphates to other proteins and are typically phospho-proteins themselves. โ By activating one kinase and introducing several different phosphotyrosines, you nucleate a molecular complex.
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