- Abnormal iron
d e p o s i t .
- A b n o r m a l
p r o t e i n
d e p o s i t i o n .
- A b n o r m a l
accumulation of
glycogen or other
carbohydrates.
- A b n o r m a l
accumulation of
f a t .
His t och e mic a l a n a l y s is
of a t i s s u e s a m p l e
al l o w s t h e pa t h o l o g i s t
to d i a g n o s e , d e t e r m i n e
th e s e v e r i t y o f t h e
di s e a s e , a n d m a k e a
pr o g n o s i s .
T h e l i g h t m i c r o s c o p e
- H em a to x y l i n a n d E o si n
- G ra m S t a i n
- G ie m sa S t a i n
- P er i od i c A c i d S c h i ff
Re a ct i on
- M as s on ' s T r i c h r o m e
- P ap a ni c o l a o u S t a i n
- N is s l S t a i n
Identification of
specific tissue
types and
identification of
abnormal tissue
Fi x a tio n
Dehydration
Embedding
Sectioning
Antigen Retrieval
Staining
ST R U C TU R E
FU N C T IO N
TI S S U E
PR E P A RA T I O N
HI S T O CH E M I ST R
Y A N D
CY T O C HE M I S TR Y
MI C R O SC O P Y
LI G H T
T h e e l e c t r o n
m i c r o s c o p e
PA T H O PH Y S I OL O G Y
CL I N I CA L
SI G N I FI C A N CE
Elaborado por
G E RA L D IN E LO R E NA R U IZ R OD R I GU E Z .