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Biotechnology is not new, because some of the techniques used in biotechnology are ancient. For example, fermentation, in which microbes are used to produce beer, wine and yogurt or agriculture, because man has chosen animals and plants from the wild and has gradually transformed them into crops and farm animals. Today researchers can take a single gene form an organism and insert him into another one of a different species. With modern biotechnology we are able to alter genes to control the production of a specific protein. With this technique, scientists have added a lot of new species. The early 70s marked the beginning of modern biotechnology, when scientist learned to alter the genetic constitution of a living organism, modifying bacteria and proteins which allowed an innovation in many fields such as medicine, with the production of antibiotics and vaccines, and agriculture, with son products that help protect endangered species.
The DNA contains genes, passed to us in equal parts by our parents. The complete DNA forms the genome that contains all genes responsible of encoding proteins and determining features of our body, such as eye colour. The DNA is a molecular chain located in chromosomes. Genes build some specific proteins that interact each other to perform most vital functions in cells. Genetic engineering is the ability to manipulate the genes of an organism to obtain a given protein and it can be used to produce variety of plants and animals with specific characteristics. Gene technology allows us to identify which gene is responsible for a particular characteristic, and it’s possible to transfer those genes into a different living organism.
In 1953, after the discovery of the DNA structure by Francis Crick and James Watson, scientists revealed that all living organisms use the same DNA code and because genes are chemically the same, genes of organisms were potentially interchangeable.
Polymerase chain reaction (PCR) uses a peace of DNA as a model to make many copies and produce a large amount of copied DNA sequence. Gel electrophoresis is a technique used to identify and separate molecules according to their size. Molecules pass through an electric field in a gel. Smaller molecules will move faster than large ones. Blotting is a technique used after molecules have passed through the electric field and have been separated by gel electrophoresis. A special absorbent material is placed on top of the gel where it picks up DNA. Restriction enzymes and ligases are naturally-occurring enzymes used to cut and join pieces of DNA. The “DNA scissors” cuts DNA at a specific known place and the “DNA glue” rejoins the DNA after cutting. Thanks to this technique, a gene can be transformed into a different DNA sequence. Gene insertion involves the insertion of new genes into the cell’s existing genetic material.
Bioremediation is the use of micro-organisms to help remove toxic chemical wastes. The environment can be cleaned by naturally-occurred enzymes or genetically-modified bacteria and fungi. Huge number of bacteria exist naturally in soil, rubbish. Some of them use oil as a nutrient and could be used to break down oil spills at sea. Biotechnology made possible the increasing of the range of treatments and with modern technology it is possible to recombine and mix genes to create a variety of bacterial species.